We narrowed to 3,985 results for: LYC;

1. SpyTag-RBD Delta

   Plasmid

   #214723

   Purpose

   Expresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

   Insert

   Receptor-Binding Domain (S SARS-CoV-2)
   

   Use

   Tags

   SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC-C Sars-CoV-2 S_L241-243del

   Plasmid

   #194841

   Purpose

   Sars-CoV-2 S_L241-243del in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   S_L241-243del (S Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   L241-243del

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pExp-His-zBasic-RBD-Avi

   Plasmid

   #195000

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   Avi-tag and His8-Zbasic

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

   Plasmid

   #170193

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   E484K

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

   Plasmid

   #170194

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   E484K

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

   Plasmid

   #170189

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Promoter

   Available since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pVD1 tRNA-gRNA E4-En-1 (GB2243)

   Plasmid

   #160565

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E4-En-1 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-sACE2-T92Q-Fc(IgA1)

   Plasmid

   #145162

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEAHISMRSATAG

    Plasmid

    #97004

    Purpose

    Expressing MRSA TAG protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    DNA-3-methyladenine glycosylase I
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-LucL

    Plasmid

    #174048

    Purpose

    Expresses mLAMA4 (VGFNFRTL) antigen as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucL
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    LAMA4 glycine 1254 to valine

    Promoter

    Human Ubiquitin C

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM5-WT PHGDH

    Plasmid

    #83901

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer10b-EGFP-KRAS G12V

    Plasmid

    #164925

    Purpose

    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 12 changed to valine (G->T 3156)

    Promoter

    Ubc Promoter and Ubc promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-∆Hapln1

    Plasmid

    #200778

    Purpose

    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Expression

    Mutation

    deleted lectican binding domain (aminoacids 40-15…

    Promoter

    Synapsin

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-HAPLN1

    Plasmid

    #200777

    Purpose

    Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Expression

    Mutation

    Promoter

    Synapsin

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer10b-HA-KRAS G12V CO SR

    Plasmid

    #164928

    Purpose

    Expresses HA tagged, codon Optimized and siRNA-resistant, tetracycline-inducible KRASG12V sequence

    Depositor

    Ji Luo

    Article

    Yuan et al Cancer Discov. 2014 Oct;4(10):1182-1197.

    Insert

    KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mutation

    Glycine 12 changed to valine; 9 Valine codons are…

    Promoter

    Ubc promoter

    Available since

    May 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1+FLAG-KrascomG12D

    Plasmid

    #206844

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-i53

    Plasmid

    #92170

    Purpose

    recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    i53
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A mutation and no terminal Glycines

    Promoter

    CMV

    Available since

    July 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4.22-PGK1-HRE::dLUC

    Plasmid

    #128095

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Mutation

    Promoter

    inserted PGK1 hypoxia response elements (HRE) (x3)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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