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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pU

Plasmid

#64180

Purpose

harbours marker gene URA3

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG34

Plasmid

#35125

Depositor

John McCusker

Publication

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

hphMX4

Use

Yeast replicating

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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EZ-L580

Plasmid

#173023

Purpose

Encodes OptoAMP4 circuit

Depositor

Jose Avalos

Publication

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pKL08

Plasmid

#104432

Purpose

pHuji in yeast expression vector

Depositor

Joel Kralj

Publication

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

pHuji

Expression

Yeast

Promoter

Tef1

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB2794

Plasmid

#63651

Purpose

pCfB2794 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB2791

Plasmid

#63654

Purpose

pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB2798

Plasmid

#63659

Purpose

pCfB2798 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Publication

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-A

Plasmid

#79160

Purpose

Expresses the Pl-A biosensor under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-A

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Pl-B

Plasmid

#79161

Purpose

Expresses the Pl-B biosensor under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Pl-B

Expression

Bacterial

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDEST1K18ms

Plasmid

#72646

Purpose

Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

Depositor

Brian Kvitko

Publication

Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMV-LEU2

Plasmid

#65334

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

LEU2

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB8740

Plasmid

#126906

Purpose

Template for PCR amplification of XdCrtI

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8741

Plasmid

#126907

Purpose

Template for PCR amplification of XdCrtE

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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POT5-TEF2p-ycrtYB-ADH1t

Plasmid

#65359

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtYB-ADH1t

Use

Unspecified

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-TEF2p-ycrtE-ADH1t

Plasmid

#65353

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtE-ADH1t

Use

Unspecified

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pBN63

Plasmid

#35104

Depositor

John McCusker

Publication

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1PR

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Publication

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pGL0_142 [EilR-PjexD]

Plasmid

#199026

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

EilR-PjexD

Use

Synthetic Biology

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCfB8742

Plasmid

#126908

Purpose

Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

pGPD1 and pFBA1

Use

Synthetic Biology

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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