We narrowed to 21,839 results for: Tes

1. pDONR223_NKX2-1_V205F

   Plasmid

   #221473

   Purpose

   Gateway cloning of NKX2-1 V205F DNA binding mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   V205F

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX-EF1a_NKX2-1_V205F

   Plasmid

   #221485

   Purpose

   NKX2-1 V205F DNA binding mutation lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   V205F

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLX-SFFV_NKX2-1

   Plasmid

   #221496

   Purpose

   NKX2-1 short isoform lentiviral overexpression using SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKJ223

   Plasmid

   #217497

   Purpose

   Bacterial expression of BaFnuc guide RNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   BaFnuc guide RNA scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKJ224

   Plasmid

   #217496

   Purpose

   Bacterial expression of BaFnuc for IVTT

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   BaFnuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKJ235

   Plasmid

   #217493

   Purpose

   Mammlian expression of MmFnuc for genome editing

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   MmFnuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

   Plasmid

   #208625

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 72~197, other residues not included

   Promoter

   T7

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUdO1s

   Plasmid

   #210238

   Purpose

   high-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUdO2te

   Plasmid

   #210243

   Purpose

   medium-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKJ318

    Plasmid

    #217495

    Purpose

    Mammlian expression of MmFnuc guide RNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    MmFnuc guide RNA scaffold
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUdOanc-a

    Plasmid

    #210320

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKJ234

    Plasmid

    #217492

    Purpose

    Mammlian expression of DpFnuc for genome editing

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    DpFnuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKJ221

    Plasmid

    #212319

    Purpose

    Express MmFnuc guide with pureexpress

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    Mercenaria guide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUdO2t

    Plasmid

    #210247

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUdO1t

    Plasmid

    #210246

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUdO2s

    Plasmid

    #210239

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUdO2k

    Plasmid

    #210235

    Purpose

    medium-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKJ238

    Plasmid

    #212321

    Purpose

    Express KnFnuc guide with pureexpress

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    KnFunc guide
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUdO4te

    Plasmid

    #210245

    Purpose

    low-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUdO1te

    Plasmid

    #210242

    Purpose

    high-copy plasmid for bacterial expression

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXOOY

    Plasmid

    #196449

    Purpose

    A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

    Depositor

    Per Amstrup Pedersen

    Article

    Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

    Type

    Empty backbone

    Use

    Expression in xenopus laevis

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    yeast CYC-GAL and T7 promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. IAA17-4flag-HIS5

    Plasmid

    #207015

    Purpose

    For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ClhN-HIS5

    Plasmid

    #207014

    Purpose

    Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ClhN-MET15

    Plasmid

    #207013

    Purpose

    Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ClhN-URA3

    Plasmid

    #207012

    Purpose

    Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RPO41 deltaN100

    Plasmid

    #205073

    Purpose

    RPO41 protein expression

    Depositor

    Smita Patel

    Article

    Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

    Insert

    RPO41 (RPO41 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N Terminal 100 amino acids

    Promoter

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETSUMOSyt1C2AB(80-421)

    Plasmid

    #209005

    Purpose

    Recombinant protein expression of truncated syt1 construct

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid

    #208644

    Purpose

    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.

    Depositor

    Quanfu Ma

    Insert

    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    

    Use

    CRISPR; Ssdna donnor preparation

    Tags

    Twin Strep

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-msEGFP1.8

    Plasmid

    #208619

    Purpose

    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.

    Depositor

    Quanfu Ma

    Insert

    msEGFP1.8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (compared with pEGFP-C1, protein level) S30R, Y39…

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKJ217

    Plasmid

    #212318

    Purpose

    Express MmFnuc with pureexpress

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    Mercenaria
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge