We narrowed to 4,253 results for: biorxiv
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Plasmid#186740PurposeDox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorUseLentiviralTagsRAB10-T2A-RABIF-P2A-mCherryExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only
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anti-VGluT1 scFv [N28/9] with sortase tag
Plasmid#204424PurposeMammalian Expression of anti-VGluT1 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N28/9.DepositorInsertRecombinant mouse scFv targeting VGluT1 (Rattus norvegicus)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-PSD-95 scFv [K28/43] with sortase tag
Plasmid#204422PurposeMammalian Expression of anti-PSD-95 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K28/43.DepositorInsertRecombinant mouse scFv targeting PSD-95 (Homo sapiens)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10APromoterU6 and CAGAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
Plasmid#225698PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK27MPromoterpEF1AAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
anti-GFP scFv [N86/38] with sortase tag
Plasmid#204419PurposeMammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.DepositorInsertRecombinant mouse scFv targeting GFP (Aequorea victoria)
UseTags6xHis tag and Sortase tagExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)
Plasmid#223167Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcdna 3.1 DSG-3 Tension Sensor
Plasmid#182717Purposeexpresses DSG-3 TSmodDepositorInsertdesmoglein-3 (Dsg3 Mouse)
UseTagsExpressionMammalianMutationTSmod inserted between G726 and G727PromoterAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTUU
Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mEtfdh CRISPR_2
Plasmid#232313PurposeMouse Etfdh Gene Knockout (gRNA 2)DepositorInsertEtfdh-targeting gRNA (Etfdh Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEtfdh CRISPR_1
Plasmid#232312PurposeMouse Etfdh Gene Knockout (gRNA 1)DepositorInsertEtfdh-targeting gRNA (Etfdh Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC84_HBB(HBA1reg-HBA1)
Plasmid#232410PurposeAAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)
Plasmid#232411PurposeAAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC62_HBB(HBA1reg-HBA1-2A-YFP)
Plasmid#232408PurposeAAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC83_HBB(HBBreg-HBA1)
Plasmid#232409PurposeAAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.DepositorInsertHemoglobin Subunit Alpha 1 (HBA1 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima)
Plasmid#223794PurposeStable expression of protein in mammalian cell cultureDepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A; deltaLIR3)
Plasmid#223775PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
UseTagsEGFP-TEV-GSTExpressionBacterialMutationI224A/L227A; deletion of LIR3PromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I307A/V310A; deltaLIR4)
Plasmid#223776PurposeExpression of recombinant protein for purificationDepositorInsertBCL2L13 soluble part (BCL2L13 Human)
UseTagsEGFP-TEV-GSTExpressionBacterialMutationI307A/V310A; deletion of LIR4PromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)
Plasmid#223777PurposeExpression of recombinant protein for purificationDepositorInsertBNIP3 soluble part (BNIP3 Human)
UseTagsThrombin cleavage-GSTExpressionInsectMutationE44A L47A D49A A50K Q51A; disrupts WIPI2 bindingPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only