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pNLF1-C_RAB7A:1-207

Plasmid

#211098

Purpose

Mammalian expression of full-length human RAB7A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RAB7A:M1-C207 (RAB7A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_AAMP:1-434

Plasmid

#211265

Purpose

Mammalian expression of full-length human AAMP. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

AAMP:M1-R434 (AAMP Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_mApple-SEPT7 Gmut2

Plasmid

#180317

Purpose

mammalian expression of human SEPT7 Gmut2 fused to mApple

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-R536C-WLS-HA-IRES-GFP

Plasmid

#178065

Purpose

Expression of WLS-HA protein (R536C mutant)

Depositor

Joseph Gleeson

Publication

Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

Insert

WLS (WLS Human)

Tags

HA and IRES-GFP

Expression

Mammalian

Mutation

R536C

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_EEF1A1_GTP-EFTU-D2

Plasmid

#109811

Purpose

Protein expression and purification of EEF1A1_GTP-EFTU-D2

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EEF1A1_GTP-EFTU-D2 (EEF1A1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Arl6ip6

Plasmid

#120235

Purpose

Expresses V5-tagged Arl6ip6 in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Arl6ip6 (Arl6ip6 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

Plasmid

#112839

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Publication

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

SH3 (N-term) domain of Grb2

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hAR-1-660

Plasmid

#89110

Purpose

mammalian expression of human androgen receptor amino acid residues 1-660, insert can be excised with EcoRI/Xba or BamHI

Depositor

Elizabeth Wilson

Publication

Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

Insert

hAR-1-660 (AR Human)

Expression

Mammalian

Mutation

contains aa 1-660

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
Rat SERCA3 (pMT2)

Plasmid

#75185

Purpose

Mammalian expression of ATP2A3

Depositor

Jonathan Lytton, Gary Shull

Publication

Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

Insert

ATP2A3 (Atp2a3 Rat)

Expression

Mammalian

Promoter

adenovirus major late promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-Zyxin-6

Plasmid

#57802

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Zyxin (ZYX Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Tubulin-6

Plasmid

#55097

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
PA-mCherry-miniSOG-Peroxisomes-2

Plasmid

#54847

Purpose

Localization: Peroxisomes, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PA-mCherry-miniSOG-Peroxisomes

Tags

Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pEMS1162

Plasmid

#29077

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple94 (GPR88 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available since

Aug. 3, 2011

Availability

Academic Institutions and Nonprofits only

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XE9 Xwnt-8 CSKA

Plasmid

#16866

Depositor

Randall Moon

Publication

Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Insert

Xwnt-8 (wnt8a.L Frog)

Use

Xenopus expression

Tags

myc?

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
xfz7N/pCS2+

Plasmid

#16325

Depositor

Peter Klein

Publication

Deardorff et al Development. 1998 Jul . 125(14):2687-700

Insert

frizzled-7 (fzd7.L Frog)

Use

Vertebrate expression

Mutation

Amino terminal end

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pGFP Glra2 KA (CC#303)

Plasmid

#15201

Depositor

Connie Cepko

Publication

Young et al Neuron. 2004 Mar 25. 41(6):867-79.

Insert

Glra2 (GLRA2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K310A

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-SpyTag-Flag-SRSF3-V5-mCherry

Plasmid

#235091

Purpose

SRSF3 mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Publication

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-SRSF3-V5-mCherry (SRSF3 Human)

Expression

Mammalian

Promoter

CAG

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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