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Plasmid#227754PurposeExpresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
UseTagsExpressionYeastMutationLeu87IlePromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_FG
Plasmid#217595PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_AF
Plasmid#217593PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_FG
Plasmid#217585PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_pBR322_GH
Plasmid#217596PurposeDestination vector with kanamycin resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_AF
Plasmid#217583PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_AE
Plasmid#217582PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_GH
Plasmid#217586PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVK_p15A_EF
Plasmid#217584PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_SS9sp
Plasmid#212145PurposeEffector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only