...meet...  Hmmm, they need a name. And we need your help in finding a good name for Blugene’s virus friend... As with Blugene’s name, we’d like to give their virus friend a gender neutral name. The Addgenies will...  During the month of January, we’ll be taking name suggestions using this form or the hashtag #BlugeneVirusFriend...finalists and we’ll ask you to vote for your favorite name. We hope you submit your best ideas because the ...newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional ...

...pXXXXX-XXXX Step 1: Backbone name Include the empty backbone name in your plasmid name. This simple piece of ...Database. Step 2: Insert name  Include information about the insert in your plasmid name. This is often a 3-...There are no universal rules for naming plasmids but here are some good guidelines to follow in order..." is often used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid...modification, this should be included in the plasmid name. Mutations are generally listed as the amino acid...either directly before or directly after the insert name.   Example: If the Glutamine at position 295 was...and often how it can be used just by reading the name. For more information about plasmid features and...

...conference room names. The Addgenie’s favorite conference room naming scheme? Gene names! This is not surprising...surprising for Addgene. But the names we chose were not just any gene names. Now, we have meetings in rooms...Bagpipe. How does a gene get its name? Often times, a gene gets its name based on what happens to an organism...conference room names originated from. Let’s take a look at some of the stories behind the names. We also have...communities have a little more leeway in what they can name a gene and many of the interesting ones come from...actually used as a break room for Addgenies, was named after the protein Groucho, a transcriptional co-...the comedian Groucho Marx, so the gene was aptly named groucho (Fisher and Caudy, 1998). Interested in ...

...quantitative real-time PCR (qPCR). Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. J. Vis. Exp . 2012...

...SOD1 C57D/C146D SOD1 CMV ALS Nam-Chul Ha 191849 pProEx-HTa-SOD1 WT SOD1 ALS Nam-Chul Ha 191850 pProEx-HTa-SOD1...pProEx-HTa-SOD1 C57A/C146A SOD1 ALS Nam-Chul Ha 191851 pProEx-HTa-SOD1 C57D/C146D SOD1 ALS Nam-Chul Ha 191852 pProEx-HTa-SOD1...pProEx-HTa-SOD1 C6A/C111A SOD1 ALS Nam-Chul Ha 193056 pADC10-SETXhel SETX Flag CMV, p10 ALS, Ataxia with neuropathy...Promoter CMV T7 polH GAL Other Clear Filters ID Plasmid Name Gene Tags Promoter Disease PI 1177 p416 25Q GPD ...

..., Myers, M. T., Zhou, X., Hou, Z., Lozen, M. L., Nam, K. H., Zhang, Y., & Ke, A. (2024). Exploiting activation... a type? That which we call CRISPR, by any other name would…probably still edit genomes. Understanding...between two types, the Cas effector may have the same name but be completely different! They are merely linked...homology-directed repair. There are 10 subtypes of type V, named A–I and U. V-A is the most popular Cas12 effector...

...common antibody applications. Intro to the Lab Bench Name Description (Link opens in a new window) Link to...laboratory materials on a balance Basic Molecular Biology Name Description (Link opens in a new window) Link to...or cells without a commercial kit Plasmid Cloning Name Description (Link opens in a new window) Link to...consortium for targeting human and mouse genes. Virus Name Description Link to Video General Transfection Introduce... titer of your lentivirus preparation Antibodies Name Description Link to Video Transfection Introduce...

...the sample name, experiment type, supermix type (ddPCR Supermix for Probes), the target names and target...

...the sample name, experiment type, supermix type (ddPCR Supermix for Probes), the target names and target...

...imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine dynamics with designed ...biosensor by name, target, publication title or keywords (e.g. "RCaMP", "caspase", "Dynamic Visualization...Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20...erGAP3 (GFP-Aequorin Protein) for imaging of Ca+ dynamics in endoplasmic reticulum GFP-Aequorin Protein ...Protein Sensor for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun...fluorescent CEPIA indicators for visualization of Ca(2+) dynamics in mitochondria. Sci Rep. 2020 Feb 18;10(1):2835...ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science. 2017 Nov 3;358(...

..., see our Guide to Plasmid Pooled Libraries . ID Name Description Genes/Insert Selection PI Human knockout...or cuts ) or single-stranded breaks ( nicks ). ID Name Insert Function Antibiotic Resistance Activity PI...transactivator and used as a transcriptional activator . ID Name Insert Antibiotic Resistance Activity PI 61422 dCAS9...-Blast-WPRE) Zhang Pooled Barcoding Libraries ID Name Description PI 115643 Morris CellTag Barcode Library...index cells for single-cell analysis of clonal dynamics. This library expresses EGFP for easy visualization...

...in a new window) . Browse Available PHP.eB AAV ID Name Promoter Description Category PI Controls 28306 ...Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter Description Category PI 28306 pAAV-FLEX-tdTomato...Addgene #127847) . Browse Available PHP.V1 AAV ID Name Promoter Description Category PI 104052 pAAV-CAG-DIO-EYFP...Addgene #185136) . Browse Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #185137) . Browse Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-...

.... Label the bottom of the plate with the plasmid name and the date. It is also a good idea to add the ...

...µg/mL Coumermycin 25 µg/mL Gentamycin 10 µg/mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10...

...information about these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40...AAV.TBG.PI.Cre.rBGe TBG none 8 Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1...phSyn1(S)-DreO-bGHpA Syn none 5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1...none 1, rg* Janelia Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-...pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre...

...laboratories must be clearly marked as “BSL-2.” The names and contact information of the laboratory manager...

...MTNR1B melatonin receptor 1B MEL-1B-R, MT2 NAMPT nicotinamide phosphoribosyltransferase 1110035O14Rik, ...go to the gene table for each category. The gene names in these tables link to the available plasmids for...differentiation, and apoptosis. Plasmid Tables The gene names in these tables link to the available plasmids for...II molecules found on the surface of APC. Symbol Name Synonyms ADRM1 adhesion regulating molecule 1 ARM1...avidity BCR through repeat cycles of expansion. Symbol Name Synonyms AKT1 v-akt murine thymoma viral oncogene...sites of inflammation throughout the body. Symbol Name Synonyms C3 complement component 3 ARMD9, ASP, CPAMD1...the immune response to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB...

...encounter: the compound light microscope. As the name suggests, light microscopes take advantage of the...

...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA...purification (which can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a...experiments, including neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV...encode tools that can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP...

... proliferation. mTOR Pathway Plasmids Click on a name to find available plasmids for the gene, or browse...Return to top mTOR Pathway - Gene List Click on a name to find available plasmids for the gene. For components...gene page are provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E...tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine thymoma viral oncogene...