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Plasmid#73217PurposeBacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Schistosoma japonicum, Human, Synthetic)
UseTags10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG
Plasmid#229223PurposeFor expression of T. elongatus KaiB protein mutantDepositorInsertkaiB (kaiB T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutation1-94 (truncated), Y8A, Y94APromoterAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y
Plasmid#226349PurposeExpression of Titin V15P substrate with GS tailDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR
Plasmid#169135PurposeE. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.DepositorInsertCoh7
UseTags3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…ExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-191~307-mEGFP(A206K)-TwStrep
Plasmid#208626Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 191~307, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-299~398-mEGFP(A206K)-TwStrep
Plasmid#208627Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 299~398, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-212~285-mEGFP(A206K)-TwStrep
Plasmid#208629Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 212~285, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis
Plasmid#175788PurposePlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis
Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only