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We narrowed to 9,597 results for: EPO

Showing: 9201 - 9250 of 9597 results

  1. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  2. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

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  3. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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  4. pAAV.hSyn.FLEX.iGluSnFR3.v857.IgK-NGR

    Plasmid
    #196226
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in NGR backbone. iGluSnFR3.v857.NGR
    Depositor
    Kaspar Podgorski
    Article
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV hSyn FLEX iGluSnFR3 v857.NGR
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    IgK-chain, Myc epi tag, and NGR TM Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196226-plasmid-map-sequence-id-409162

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  5. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  6. AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128589
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV8
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    Syn
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128589-plasmid-map-sequence-id-257684

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  7. pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH]

    Plasmid
    #196885
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Frank Bradke
    Article
    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Quimeric CAG
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    196885-plasmid-map-sequence-id-391918

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  8. pAAV-hSyn-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

    Plasmid
    #220610
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220610-plasmid-map-sequence-id-441133

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  10. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #84445
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84445-plasmid-map-sequence-id-177041

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  12. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

    Plasmid
    #128387
    Purpose
    Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc398-3xGGGGS-LGALS8 (LGALS8 Synthetic, Human)
    CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    CLuc394 and NLuc398
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128387-plasmid-map-sequence-id-261539

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  14. pIS1 IL6R

    Plasmid
    #60796
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and wild-type miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and wild-type miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60796-plasmid-map-sequence-id-97807

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  15. pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

    Plasmid
    #179431
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179431-plasmid-map-sequence-id-362554

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  16. STARR-seq luciferase INF enhancer vector_ORI_ISG15

    Plasmid
    #99322
    Purpose
    Luciferase validation vector with ISG15 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr1: 947976 -949995 (ISG15 Human)
    Use
    Luciferase; Starr-seq luciferase validation vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    99322-plasmid-map-sequence-id-190934

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  23. pEMS1503

    Plasmid
    #29299
    Purpose
    Homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ reporter.
    Depositor
    Elizabeth Simpson
    Article
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Insert
    Ple32 (CLDN5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29299-plasmid-map-sequence-id-13714

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  25. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

    Plasmid
    #122103
    Purpose
    AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Insert
    Chronos-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a（1.1kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122103-plasmid-map-sequence-id-256678

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  30. pLY164

    Plasmid
    #184140
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R3, PrhaB, and Ptet
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184140-plasmid-map-sequence-id-378329

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  31. pLY305

    Plasmid
    #184159
    Purpose
    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    K140N, Mutations D10A and H840A on WT cas9. Synon…
    Promoter
    Pcon, PpspA-zraP-22, PrhaB, and Ptet
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184159-plasmid-map-sequence-id-376083

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  32. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid
    #173963
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    Use
    Luciferase
    Tags
    Expression
    Mutation
    1 mutated Coordinator motif
    Promoter
    SV40 promoter
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    173963-plasmid-map-sequence-id-341768

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  34. pGL3E-hGPR56 e1m promoter normal

    Plasmid
    #52298
    Purpose
    Reporter for normal human GPR56 exon 1m promoter activity. Human GPR56 e1m promoter (chr16:56,230,630-56,230,943 (hg18)) was inserted into pGL3E.
    Depositor
    Christopher A Walsh
    Article
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Insert
    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52298-plasmid-map-sequence-id-80883

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  37. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    130923-plasmid-map-sequence-id-257307

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  38. pIS1 mIL6R

    Plasmid
    #60797
    Purpose
    Luciferase reporter to measure miR-155 mediated differential repression. Contains IL6R 3' UTR and mutated miR-155 sites
    Depositor
    David Bartel
    Article
    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi
    Insert
    IL6R 3'UTR and mutated miR-155 binding site (IL6R Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    mutated miR-155 binding site
    Promoter
    thymidine kinase (HSV-TK promoter)
    Available since
    Oct. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    60797-plasmid-map-sequence-id-97808

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  39. pEMS1401

    Plasmid
    #29198
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Expression
    Mutation
    Promoter
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29198-plasmid-map-sequence-id-13613

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  41. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid
    #50483
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Use
    Tags
    YFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the YFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pDONR P2R-P3-IRES_ECFP

    Plasmid
    #48354
    Purpose
    Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    IRES_ECFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    ECFP translation is mediated by an IRES. Contains…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48354-plasmid-map-sequence-id-70631

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  44. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid
    #52299
    Purpose
    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.
    Depositor
    Christopher A Walsh
    Article
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Insert
    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    15-bp deletion in the promoter
    Promoter
    Human GPR56 e1m promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52299-plasmid-map-sequence-id-80926

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  45. pCDNA3.1-nAChr-beta3-CFP

    Plasmid
    #50485
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    CFP fusion in M3-M4 loop (after residue P379). O…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  46. pEMS1538

    Plasmid
    #29261
    Purpose
    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving a lacZ reporter
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119
    Insert
    Ple67 (FEV Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Expression
    Mutation
    Promoter
    Available since
    Oct. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29261-plasmid-map-sequence-id-13676

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  47. pAAV-hSyn T21R(I18R+M72E)-F8.2(S54L+T127A)-T2A-mCherry

    Plasmid
    #225591
    Purpose
    AAV transgene plasmid with hSyn promoter for expression of catalytically dead TRIM21 RING(I18R+M72E)-F8.2(S54L+T127A) anti-tau degrader with a self-cleaving T2A-mCherry fluorescent expression reporter
    Depositor
    William McEwan
    Article
    Benn et al Science. 2024 Aug 30;385(6712):1009-1016
    Insert
    T21R(I18R+M72E)-F8.2(S54L+T127A)-T2A-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    225591-plasmid-map-sequence-id-446528

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  49. pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

    Plasmid
    #220609
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.
    Depositor
    Sam Golden
    Article
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220609-plasmid-map-sequence-id-441116

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  50. pcs2-MTSmut-cox8A(XXXX)-GFP-IRES-mCherry

    Plasmid
    #226104
    Purpose
    Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) with mutations that impair recognition by SIFI for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:
    Insert
    COX8A (COX8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    encodes only for mitochondrial targeting sequence…
    Promoter
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    226104-plasmid-map-sequence-id-448409

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Showing: 9201 - 9250 of 9597 results