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pTol2EF1a-BglobinIntron-sfGFP-Fb(BC2)

Plasmid

#249319

Purpose

psfGFP-Fb(BC2) expression in vertabrate cells. pTol2 vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Methods. 2026 Apr 22. doi: 10.1038/s

Insert

sfGFP-Fb(BC2)

Use

Other

Promoter

EF1a

Available Since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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sh-hCHIP

Plasmid

#246751

Purpose

Mammalian and lentiviral expression of shRNA targeting human CHIP (Stub1)

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

sh-hCHIP (STUB1 Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB082

Plasmid

#239578

Purpose

Plant RNA Vision constructs with 41 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA01
gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB083

Plasmid

#239579

Purpose

Plant RNA Vision constructs with 82 bp gRNAs together with RFP input

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Inserts

sfGFPn_Y66-P1_loop_01-stem01-gRNA03
gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP

Use

Synthetic Biology

Expression

Plant

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pRSETB

Plasmid

#236200

Purpose

Bacterial expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Bacterial

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pJethlpAmNeonGreenCm

Plasmid

#206814

Purpose

Template for hlpA-mneongreen amplification associated with chloramphenicol resistance gene

Depositor

Virginie Libante

Article

Mignolet et al Microlife. 2026 Feb 26;7:uqag006. doi: 1

Insert

hlpA

Use

Synthetic Biology

Tags

mNeonGreen

Mutation

3insG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway Cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-TAEL (JDW 1324)

Plasmid

#224508

Purpose

Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

TAEL

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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NBS1 C-terminal sgRNA

Plasmid

#207092

Purpose

pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

TAAAAAGGAGAAGATAACTG

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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ATM N-terminal sgRNA

Plasmid

#207089

Purpose

pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ATCATTAAGTACTAGACTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_AVR1-CO39

Plasmid

#123654

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

AVR1-CO39

Use

Golden gate

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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HCB2

Plasmid

#98583

Purpose

Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49-148 of huntingtin, fused to eYFP.

Depositor

Ray Truant

Article

Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

Insert

HCB2

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PEST2D

Plasmid

#62434

Purpose

MXS_chaining vector with PEST2D

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Destabilization tag

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-dLight3.6

Plasmid

#257856

Purpose

Cre-dependent expression of the green genetically encoded dopamine sensor dLight3.6 under synapsin promoter.

Depositor

Lin Tian

Article

Roshgadol et al Res Sq [Preprint]. 2025 Aug 20:rs.3.rs-7

Insert

dLight3.6

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-CyTERM-mTagBFP2-24xPP7

Plasmid

#254845

Purpose

an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of PP7 RNA tag

Depositor

Jiahui (Chris) Wu

Article

Pham et al Nat Methods. 2026 Jan;23(1):165-174. doi

Insert

CyTERM-mTagBFP2-24xPP7

Expression

Bacterial

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-CyTERM-mTagBFP2-24xMS2

Plasmid

#254843

Purpose

an mRNA reporter expressing with CyTERM-mTagBFP2 with 24 copies of MS2 RNA tag

Depositor

Jiahui (Chris) Wu

Article

Pham et al Nat Methods. 2026 Jan;23(1):165-174. doi

Insert

CyTERM-mTagBFP2-24xMS2

Expression

Bacterial

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM045

Plasmid

#247633

Purpose

to generate mRNA for membrane mScarlet3 for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mScarlet3

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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