We narrowed to 5,111 results for: codon optimized

1. pNHT7::RPL15::hGluc

   Plasmid

   #186768

   Purpose

   An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Gaussia luciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-FH-Nsp1

   Plasmid

   #157680

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. AAV2_hSyn_Phobos_Citrine

   Plasmid

   #98216

   Purpose

   Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC39A14

   Plasmid

   #132105

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A14 (SLC39A14 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR221_SLC7A3

   Plasmid

   #132301

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

   Insert

   SLC7A3 (SLC7A3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA5-FRT-TO-Nsp1-HF

   Plasmid

   #157681

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   HF-Nsp1 (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEGB2Ω2_SF-35s:hCas9:tNos (GB1103)

   Plasmid

   #75400

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter. Specially conceived to be linked with the TU of the kanamycin resistance gene GB1181.

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNHT7::RPL15::sNluc2

   Plasmid

   #186763

   Purpose

   An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Promoter

   T7

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nsp14D-mCherry

   Plasmid

   #165139

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp14D (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV2_hSyn_Aurora_Citrine

    Plasmid

    #98217

    Purpose

    Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV2_hSyn_iChloC_CA_Citrine

    Plasmid

    #98220

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChloC_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, C128A, T159C, D156N

    Promoter

    human synapsin

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-FRT-TO-Nsp7-HF

    Plasmid

    #157690

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

    Plasmid

    #192471

    Purpose

    Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    AcGFP1

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid

    #104568

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x1)
    

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs_FLAG-MCART1Q90A

    Plasmid

    #133249

    Purpose

    Express FLAG-tagged MCART1Q90A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, Q9…

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs_FLAG-MCART1R182A

    Plasmid

    #133252

    Purpose

    Express FLAG-tagged MCART1R182A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    MCART1 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, R1…

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid

    #111862

    Purpose

    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDN(1-400)-GFP (Bicd2 Mouse)
    

    Tags

    8xHis tag, GFP, and ZZ tag

    Expression

    Insect

    Mutation

    Codon optimized for Sf9 expression

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid

    #104569

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x2)
    

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-FH-Nsp8

    Plasmid

    #157695

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp8 (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pETM6-3F2-mCherry

    Plasmid

    #73418

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P3F2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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