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pFA6a-kanMX6-3nmt1-mEOS3.2

Plasmid

#105166

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mEOS3.2

Tags

3nmt1-mEOS3.2

Expression

Bacterial and Yeast

Promoter

3nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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BS-Trp1

Plasmid

#69196

Purpose

complementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.

Depositor

Zhiping Xie

Publication

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

TRP1

Expression

Yeast

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pRS303H-Padh-OsTIR1(F74G)

Plasmid

#247625

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

ADH1

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRS303K-Pgal-OsTIR1(F74G)-GEM

Plasmid

#247622

Purpose

Expression of OsTIR1

Depositor

Sebastian Schuck

Publication

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

OsTIR1

Expression

Yeast

Mutation

F74G

Promoter

GAL1

Available since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pIIIA/MS2-1

Plasmid

#220631

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Publication

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Publication

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHpgRNA13

Plasmid

#160127

Purpose

To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Publication

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-ADE2- HDV-TAMO

Expression

Yeast

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pHO-UAS-mScarlet-I

Plasmid

#183147

Purpose

Yeat two-hybrid fluorecent reporter for genomic integration

Depositor

Assaf Mosquna

Publication

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

mScarlet-I

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0710

Plasmid

#133517

Purpose

Expression of mCherry tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mCherry-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0761

Plasmid

#133494

Purpose

Expression of nucleo-cytoplasmic mTagBFP2 in P-cells

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mTagBFP2-terminator(ScADH1)-bleMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0770

Plasmid

#133518

Purpose

Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pK3FS-PYK1

Plasmid

#85777

Purpose

N-ICE plasmid pK3FS-PYK1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGP8A-ccdB

Plasmid

#83883

Purpose

Destination vector for integration at HIS3, expression from the ADH1 promoter

Depositor

Jennifer Nemhauser

Publication

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway Cloning

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Publication

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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DHC1-AID-Clover donor (Hygro)

Plasmid

#158622

Purpose

Donor plasmid for tagging DHC1 with AID-Clover

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR; Tagging donor

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMY.PRC2

Plasmid

#234582

Purpose

Expression of ProtA-EZH2, SUZ12, EED and RBBP4

Depositor

Chen Davidovich

Publication

Lai et al J Mol Biol. 2025 Jan 13:168943. doi: 10.

Insert

EZH2, SUZ12, EED, RBBP4 (EZH2, SUZ12, RBBP4, EED Human)

Expression

Yeast

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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sub2-1 pRS315

Plasmid

#111301

Purpose

sub2-1 in pRS315

Depositor

Christine Guthrie

Publication

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Expression

Yeast

Mutation

sub2-1

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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