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Plasmid#17199DepositorInsertbeta catenin (ctnnb1 Zebrafish)
UseTagsHISExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
M62 pPET28a-TEV-human beta cat domain N(1-137)
Plasmid#17203DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsHISExpressionBacterialMutationamino-terminal residues 1-137PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys
Plasmid#90474PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(40nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep
Plasmid#180811PurposeExpression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrepDepositorInsertGST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)
Plasmid#153441PurposeE. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode B knock-out mutant
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 140 and methionine 144 were mutated to a…PromoterT7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)
Plasmid#153440PurposeE. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode A knock-out mutant
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 191 and leucine 195 on XDocB were mutate…PromoterT7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT
Plasmid#111398PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346APromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT
Plasmid#111399PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP TagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)/I346DPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
M63 pPET28a-TEV-human beta cat domain C(666-781)
Plasmid#17204DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsHISExpressionBacterialMutationcarboxy-terminal residues 666-781PromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
Plasmid#180806PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrepDepositorInsertBTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationBTLA (Y243F, Y257F, Y282F)PromoterT7Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only