Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 6,981 results for: Mag

Showing: 5581 - 5610 of 6981 results

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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CIRC#del

Plasmid

#186344

Purpose

Contains VEGFA exon 7 and short intronic flanking regions not sufficient for expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

Plasmid

#224506

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-oMDC-LexOP-c-Fos

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

Plasmid

#224512

Purpose

A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xHA-BirA-T2A-mCherry-Caax

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

Plasmid

#224522

Purpose

A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroSCRAMBLED

Plasmid

#215594

Purpose

For overexpression of Gal4-DBD EGR1 AroSCRAMBLED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroSCRAMBLED (EGR1 Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS_PATCHED

Plasmid

#215584

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS PATCHED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS PATCHED (HOXD4 Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pNK189

Plasmid

#219743

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Homo sapiens

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK4169

Plasmid

#219744

Purpose

MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Pichia pastoris

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mutant of fungal hispidin-3-hydroxylase

Use

Synthetic Biology

Mutation

D37E, V181I, S323M, M385K

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS15+2

Plasmid

#215605

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS15+2

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15+2 (CEBPA Human)

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS

Plasmid

#215582

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS (HOXD4 Human)

Expression

Mammalian

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NGN2-AroLITE_A

Plasmid

#215608

Purpose

For overexpression of Gal4-DBD NGN2 AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 AroLITE (NEUROG2 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPERFECT_YPWM_(+)

Plasmid

#215581

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT YPWM(+)

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT YPWM(+) (HOXD4 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroLITE_G

Plasmid

#215573

Purpose

For overexpression of Gal4-DBD HOXD4 AroLITE G

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroLITE G (HOXD4 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NGN2-AroPERFECT

Plasmid

#215609

Purpose

For overexpression of Gal4-DBD NGN2 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 AroPERFECT (NEUROG2 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPERFECT_shuffled2

Plasmid

#215578

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT shuffled 2

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT shuffled2 (HOXD4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPERFECT

Plasmid

#215574

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT (HOXD4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroLITE_S

Plasmid

#215572

Purpose

For overexpression of Gal4-DBD HOXD4 AroLITE S

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroLITE S (HOXD4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroLITE_A

Plasmid

#215571

Purpose

For overexpression of Gal4-DBD HOXD4 AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroLITE A (HOXD4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-CEBPa_WT(N)-FUSN

Plasmid

#215637

Purpose

For overexpression of Gal4-DBD CEBPa WT(N) FUSN

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa(N) FUSN (CEBPA Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS15

Plasmid

#215603

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS15

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15 (CEBPA Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-HOXD4-AroPERFECT-1

Plasmid

#215575

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT-1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT-1 (HOXD4 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-CEBPa-AroPERFECT_IS15+1

Plasmid

#215604

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS15+1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15+1 (CEBPA Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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