We narrowed to 3,807 results for: golden gate

1. pML323_GOLGA2-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227910

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   GOLGA2 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227916

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   CAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227891

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   VPS35 homology arms with mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pML530_EEA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227892

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   EEA1 homology arms with mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227900

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   ATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227901

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   MAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pML349_PEX3-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227902

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   PEX3 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227904

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

   Plasmid

   #227905

   Purpose

   Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

   Depositor

   Manuel Leonetti

   Article

   Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

   Insert

   COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
   

   Use

   CRISPR

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV-U6-sgPlxnb2-KO-4-EF1a-LibVec

    Plasmid

    #239600

    Purpose

    expresses guide#4 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-U6-sgPlxnb2-KO-3-EF1a-LibVec

    Plasmid

    #239599

    Purpose

    expresses guide#3 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-U6-sgPlxnb2-KO-2-EF1a-LibVec

    Plasmid

    #239598

    Purpose

    expresses guide#2 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-U6-sgPlxnb2-KO-1-EF1a-LibVec

    Plasmid

    #239597

    Purpose

    expresses guide#1 to knock-out mouse Plxnb2, via CRISPR-Cas9

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-U6-sgPlxnb2-CRISPRa-3-EF1a-LibVec

    Plasmid

    #239596

    Purpose

    expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-U6-sgPlxnb2-CRISPRa-1-EF1a-LibVec

    Plasmid

    #239594

    Purpose

    expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-U6-sgPlxnb2-CRISPRa-2-EF1a-LibVec

    Plasmid

    #239595

    Purpose

    expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    Plxnb2 (Plxnb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sgPlxnb2-2 in pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239589

    Purpose

    expresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
    

    Use

    Sleeping beauty (sb) transposon

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgPlxnb2-3 in pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239590

    Purpose

    expresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
    

    Use

    Sleeping beauty (sb) transposon

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP

    Plasmid

    #239588

    Purpose

    expresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRa

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Insert

    sgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
    

    Use

    Sleeping beauty (sb) transposon

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi

    Plasmid

    #216166

    Purpose

    Express the gRNAs for the human FOXA1/A2/A3-CRISPRi

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA-FOXA1-Rank1, sgRNA-FOXA2-Rank2, sgRNA-FOXA2-Rank1, and sgRNA-FOXA3-Rank1 (FOXA1, FOXA2, FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    phU6, ph7SK, phH1, pmU6

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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