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FLAG-hMYOD1 (#794)
Plasmid#78329PurposeOverexpression of human MYOD1 ORF with a N-terminal FLAG tagDepositorInsertMYOD1 (MYOD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDON-AI-Npm2(N533)
Plasmid#51475PurposeExpress mouse phosphorylation-mimicking Npm2 (p-Npm, N533)DepositorInsertNucleoplasmin 2 (Npm2 Mouse)
UseRetroviralTagsExpressionMammalianMutationEleven codons (5, 6, 7, 8, 10, 82, 95, 104, 184, …PromoterAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pDual_dsCas9_Blast_hs_NC_chr1
Plasmid#214690PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Blast_hs_TP73
Plasmid#214691PurposeLentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_PROX1_1+3
Plasmid#121176PurposeLentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.DepositorInsertCas9-P2A-puro and U6 promoters driven expression of gRNAs
UseCRISPR and LentiviralTagsP2A-puroExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterPvegAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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CMV-dCas9_puroR
Plasmid#219825PurposeCMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)DepositorInsertdCas9
UseCRISPRTagsExpressionMammalianMutationD10A/H841APromoterCMVAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F3b
Plasmid#37975DepositorInsertE2F3b (E2F3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-TetRVP64
Plasmid#158133PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-TetRVP64
UseTransposonTagsExpressionMammalianMutationPromoterEF1αAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dlx6
Plasmid#99480PurposeMammalian expression of the Distal-less Homeobox 6 geneDepositorInsertDlx6 (Dlx6 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Letm1
Plasmid#191962PurposeExpression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).DepositorInsertLetm1 (LETM1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP
Plasmid#52053PurposeUsed in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.DepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromotertetR/pLtetOAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pML104-NatMx3
Plasmid#83477PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.DepositorInsertNatMx3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
SLC9A3R2-TwinStrepII
Plasmid#83200PurposeExpression of tagged protein in mammalian cellsDepositorInsertSLC9A3R2 (Slc9a3r2 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur/GDI1-splitTurboID
Plasmid#198219PurposeTransposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labelingDepositorInsertGID1
UseTransposonTags5’ part of TurboID, up to E256ExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-lamin B2
Plasmid#175109PurposeLentiviral expression of V5-tagged mouse Lmnb2DepositorInsertLmnb2 (Lmnb2 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
