We narrowed to 11,438 results for: 112

1. pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

   Plasmid

   #194697

   Purpose

   HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   elavl3(HuC)

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

   Plasmid

   #194698

   Purpose

   HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_off-mEGFP
   

   Use

   Zebrafish expression

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   elavl3(HuC)

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMX-IG-ATG3 (K62D/K64D)

   Plasmid

   #212031

   Purpose

   Expresses untagged ATG3 (K62D/K64D) in mammalian cells

   Depositor

   Michael Lazarou

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCHA1.1-COMMD3_CDS-V5-hygro

   Plasmid

   #195509

   Purpose

   lentiviral vector expressing COMMD3 CDS with hygromycin selection

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   COMMD3 (COMMD3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

   Plasmid

   #212079

   Purpose

   LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   Zmynd8+exon(48nt) (Zmynd8 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. NSP4-Δ220-234-Flag

   Plasmid

   #210341

   Purpose

   Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Δ220-234

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NSP4-H120N/F121L-Flag

   Plasmid

   #210340

   Purpose

   Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   H120N/F121L

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NSP4-FR-2Strep

   Plasmid

   #210316

   Purpose

   Expresses SARS-CoV-2 NSP4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NSP4-mCherry

   Plasmid

   #210319

   Purpose

   Expresses SARS-CoV-2 NSP4 fused with mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. STIM1-2Strep

    Plasmid

    #210361

    Purpose

    Expresses STIM1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FKBP10-EGFP

    Plasmid

    #210369

    Purpose

    Expresses FKBP10 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    FKBP10 (FKBP10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FKBP10-2Strep

    Plasmid

    #210357

    Purpose

    Expresses FKBP10 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    FKBP10 (FKBP10 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DNAJC11-2Strep

    Plasmid

    #210354

    Purpose

    Expresses DNAJC11 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    DNAJC11 (DNAJC11 Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p663-UBC-miniTurbo-V5-ZNF746-C571A

    Plasmid

    #207821

    Purpose

    N-terminal tagged ZNF746-C571A for miniTurbo proximity experiments

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    miniTurbo-V5-ZNF746-C571A (ZNF746 Human)
    

    Use

    Lentiviral

    Tags

    miniTurbo-V5

    Expression

    Mutation

    C571A

    Promoter

    UBC

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Cas9-ZNF746

    Plasmid

    #207822

    Purpose

    Dual expression of Cas9 and sgRNA targeting ZNF746

    Depositor

    Ben Major

    Article

    LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

    Insert

    ZNF746 sgRNA (ZNF746 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    EF-1a; U6

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCOA-Leu-FvPPT1

    Plasmid

    #179923

    Purpose

    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    FvPPT1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Yarrowia lipolytica EXP1

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV_PGK_LTB4R(T308A)

    Plasmid

    #208088

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A mutation.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    LTB4R(T308A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T308A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV_PGK_LTB4R(S310A)

    Plasmid

    #208089

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the S310A mutation.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    LTB4R(S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S310A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV_PGK_LTB4R(T308A,S310A)

    Plasmid

    #208090

    Purpose

    Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    LTB4R(T308A,S310A) (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T308A, S310A

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_PGK_3xHA-LTB4R

    Plasmid

    #208091

    Purpose

    Mammalian expression of the wild-type human LTB4 receptor LTB4R with an N-terminal 3x HA tag.

    Depositor

    Sean R Collins

    Article

    Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

    Insert

    3xHA-LTB4R (LTB4R Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    PGK

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCEP4-myc-EphB4

    Plasmid

    #200983

    Purpose

    Mammalian expression plasmid for myc-tagged EphB4

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBVboostFGII WPRE 3C-CVB3

    Plasmid

    #202077

    Purpose

    Bacterial expression of 3C protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB3 3C protease with HisTag
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    polh, CAG, T7

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBVboostFGII WPRE 2A-CVB3

    Plasmid

    #202076

    Purpose

    Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    CVB3 2A protease with HisTag
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    polh, CAG, T7

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. His-ATG3-H262A

    Plasmid

    #202782

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    H262A

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. His-ATG3-T244A

    Plasmid

    #202781

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    T244A

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. His-ATG3-Y209A

    Plasmid

    #202779

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Y209A

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. His-ATG3-Y210A

    Plasmid

    #202780

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    Y210A

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. His-ATG3-K208D

    Plasmid

    #202785

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    K208D

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His-ATG3-C264A

    Plasmid

    #202778

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    C264A

    Promoter

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His-ATG3-R265A

    Plasmid

    #202783

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    R265A

    Promoter

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. His-ATG3-H266A

    Plasmid

    #202784

    Purpose

    ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

    Depositor

    James Hurley

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Mutation

    H266A

    Promoter

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPB-NeoCOVGT3-d2EGFP-mCherry

    Plasmid

    #201450

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT3_d2EGFP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPB-Neo-COVGT1-d2EGFP-mCherry

    Plasmid

    #201451

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT1_d2EGFP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPB-Neo-COVGT2-d2EGFP-mCherry

    Plasmid

    #201452

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT2_d2EGFP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPB-Neo-COVGT4-d2EGFP-mCherry

    Plasmid

    #201453

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT4_d2EGFP-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPB-Neo-COVGT5-d2EGFP-mCherry

    Plasmid

    #201454

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT5_d2EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBA407-Neo-COVGT5-mCherry

    Plasmid

    #201455

    Purpose

    Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

    Depositor

    Gaetano Gargiulo

    Article

    Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

    Insert

    COVGT5_mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pENTR221-IGFBP2-Clover

    Plasmid

    #191007

    Purpose

    Gateway entry vector with IGFBP2 tagged with Clover

    Depositor

    Johanna Ivaska

    Article

    Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

    Insert

    Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)
    

    Use

    Gateway entry vector

    Tags

    Clover

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LENTICRISPR-NDUFB5_sgRNA1

    Plasmid

    #201606

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    NDUFB5 (NDUFB5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LENTICRISPR-NDUFB5_sgRNA2

    Plasmid

    #201607

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    NDUFB5 (NDUFB5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GB1-PRD(703-868, P801G)

    Plasmid

    #180029

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28a LZ control-FLAG

    Plasmid

    #199678

    Purpose

    Bacterial expression of Leucine Zipper (LZ) control-Flag

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    Leucine zipper control
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S

    Plasmid

    #147461

    Purpose

    Mammalian Expression of HsSmg7-G100E-siRNAres-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three silent mutation G216A, T1185C and A1521G an…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S

    Plasmid

    #147462

    Purpose

    Mammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three silent mutation G216A, T1185C and A1521G an…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCIneo-lambdaN-HA-HsSmg7-T103E-siRNAres_S

    Plasmid

    #147464

    Purpose

    Mammalian Expression of HsSmg7-T103E-siRNAres-shRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg7-T103E-siRNAres-shRNAres (SMG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three silent mutation G216A, T1185C and A1521G an…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pN1-HsSmg5-Q123E-siRNAres-V5-SBP-MBP_S

    Plasmid

    #147471

    Purpose

    Mammalian Expression of HsSmg5-Q123E-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

    Insert

    HsSmg5-Q123E-siRNAres (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three non silent mutation A254G (K85R), T980C (L3…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid

    #191336

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    motor domain aa 1-405

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX-4T-1-ε ear

    Plasmid

    #196670

    Purpose

    Expresses GST-AP-4 ε ear (residues 840-1137) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 ε ear (840-1137) (AP4E1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    silent mutations in codons 905, 1129 and 1137

    Promoter

    tac

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pHEN-CoV2-nanobody-C7

    Plasmid

    #194591

    Purpose

    Bacterial expression of C7 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    C7 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid

    #191335

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge