We narrowed to 3,059 results for: ALC

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pStorage_Ptet-1_ZF1

Plasmid

#213844

Purpose

CombiGEM storage plasmid for ZF-1 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pStorage_Ptet-2_ZF2

Plasmid

#213845

Purpose

CombiGEM storage plasmid for ZF-2 CR fusions.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLEU

Plasmid

#213846

Purpose

LEU integrating plasmid backbone used to create all of the reporter plasmids.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_TetR

Plasmid

#213847

Purpose

Facilitates genomic integration of tetR expression cassette.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

TetR

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pT3TS-Tol2

Plasmid

#31831

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Use

Cloning vector

Promoter

Lac

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET-GCaMP6f

Plasmid

#67564

Purpose

bacterial expression of a fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-BRIC

Plasmid

#172341

Purpose

AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS09

Plasmid

#67160

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS09

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

L414T within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP6f-RS06

Plasmid

#67159

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Badura et al Neurophotonics. 2014 Oct;1(2):025008.

Insert

Fast-GCaMP6f-RS06

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

M374Q within GCaMP6f

Promoter

T7

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBScMXT + dSlo

Plasmid

#16173

Depositor

Larry Salkoff

Article

Wei et al Neuron. 1994 Sep . 13(3):671-81.

Insert

Drosophila melanogaster calcium activated potassium channel (slo Fly)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pa-G-GECO1.2

Plasmid

#67622

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

pa-G-GECO1.2

Expression

Mammalian

Mutation

V81H, M84K, L189F, T190S

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pIM35

Plasmid

#74644

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and URA3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry

Plasmid

#230062

Purpose

Expression of genetically encoded calcium chelator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

SpiCee

Use

Zebrafish expression

Tags

FLAG and mRFP1

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_mNeonGreen

Plasmid

#213838

Purpose

Facilitates genomic integration of the control reporter without zinc finger binding sites.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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