We narrowed to 14,883 results for: pET

1. pET28b-M1

   Plasmid

   #225734

   Purpose

   To express M1 (13-193) in E. coli

   Depositor

   Nicole Sampson

   Article

   Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

   Insert

   mce3R
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   aa 13-193

   Promoter

   Available since

   Oct. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET27b+R

   Plasmid

   #188245

   Purpose

   Empty backbone vector for protein expression in Escherichia coli and his-tag purification

   Depositor

   Yiping Qi

   Article

   Fang et al Methods Mol Biol. 2023;2653:153-171. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET24a_SMOX

   Plasmid

   #186345

   Purpose

   C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

   Insert

   Spermine oxidase (SMOX Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28_LmFPPS

   Plasmid

   #186322

   Purpose

   pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

   Depositor

   Sandra Gabelli

   Article

   Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

   Insert

   FPPS
   

   Use

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET50delCT_Vipp1_dL10Ala

   Plasmid

   #223817

   Purpose

   Bacterial expression of Vipp1_dL10Ala with C-terminal 6x His-Tag and 3C cleavage site

   Depositor

   Carsten Sachse

   Article

   Junglas et al Nat Struct Mol Biol. 2025 Mar;32(3):555-

   Insert

   Vipp1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   replacement of 10 aa in Hinge 2 with Ala

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a:ChrH

   Plasmid

   #225084

   Purpose

   Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrH
   

   Use

   Tags

   His6-Thrombin

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a:ChrI

   Plasmid

   #225085

   Purpose

   Expresses anthrone-oxygenase like enzyme ChrI in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrI
   

   Use

   Tags

   His6 and His6-Thrombin

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 300_pETcon_SARS2_FLip

   Plasmid

   #222230

   Purpose

   yeast surface display of the SARS-CoV-2 FLip variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

   Insert

   SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 299_pETcon_SARS2_EG-5

   Plasmid

   #222232

   Purpose

   yeast surface display of the SARS-CoV-2 EG.5 variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

   Insert

   SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28Man

    Plasmid

    #203399

    Purpose

    exo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymes

    Depositor

    David Vocadlo

    Article

    Deng et al Bioorg Med Chem. 2013 Aug 15;21(16):4839

    Insert

    man2A
    

    Use

    Tags

    H6

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETURALEU

    Plasmid

    #223207

    Purpose

    Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

    Depositor

    Sahand Jamal Rahi

    Article

    Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a_PA1472_Cterm_His

    Plasmid

    #220485

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_speG_Nterm_His

    Plasmid

    #220478

    Purpose

    heterologous expression for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    speG
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_PA4114_Nterm_His

    Plasmid

    #220479

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_PA1472_Nterm_His

    Plasmid

    #220480

    Purpose

    heterologous expression of PA1472 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1472
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a_PA1377_Nterm_His

    Plasmid

    #220481

    Purpose

    heterologous expression of PA1377 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA1377
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OoH_pET29b

    Plasmid

    #215424

    Purpose

    Expression construct for OoH gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    OoH
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ph-NylC_pET28a

    Plasmid

    #215425

    Purpose

    Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Ph-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ReU_pET29b

    Plasmid

    #215426

    Purpose

    Expression construct for ReU gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    ReU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TtA_pET29b

    Plasmid

    #215432

    Purpose

    Expression construct for TtA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    TtA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge