Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,045 results for: golden gate

Showing: 3781 - 3800 of 4045 results

Results per page:

Sort results by:

pMBS-846

Plasmid

#218001

Purpose

3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xFLAG-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-847

Plasmid

#218002

Purpose

Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Myc-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMBS-851

Plasmid

#218006

Purpose

Spacer to bridge position B3

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Spacer

Use

Synthetic Biology

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS2

Plasmid

#184885

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS3

Plasmid

#184886

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pECNUS1

Plasmid

#184884

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-mini v2-HF24_F4

Plasmid

#195718

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#4 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F20

Plasmid

#195734

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#20 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F12

Plasmid

#195726

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#12 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F3

Plasmid

#195717

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#3 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F24

Plasmid

#195738

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#24 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F7

Plasmid

#195721

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#7 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F8

Plasmid

#195722

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#8 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F14

Plasmid

#195728

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#14 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F13

Plasmid

#195727

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#13 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F22

Plasmid

#195736

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#22 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F10

Plasmid

#195724

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#10 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pUC57-mini v2-HF24_F6

Plasmid

#195720

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#6 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

Feb. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 3781 - 3800 of 4045 results

Results per page:

Sort results by: