We narrowed to 3,807 results for: golden gate

1. pSEED-mGreenLantern

   Plasmid

   #219643

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. JME4599

   Plasmid

   #129660

   Purpose

   NATex_CrisprCas9-yl_RFP: CAS9 vector with Nourseothricin ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Level 1 P4 TaU6 guide acceptor

   Plasmid

   #165600

   Purpose

   GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Level 1 P3 TaU6 guide acceptor

   Plasmid

   #165599

   Purpose

   GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

   Depositor

   John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

   Article

   Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

   Insert

   TaU6 promoter::LacZ::sgRNA scaffold
   

   Use

   CRISPR and Synthetic Biology; Sgrna acceptor with…

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ttAtCas12a+int

   Plasmid

   #182384

   Purpose

   Gives very high editing efficiency in barley. Also works in Brassica oleracea

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   LbCas12a coding sequence with D156R and Arabidopsis introns
   

   Use

   CRISPR

   Mutation

   D156R + 8 Arabidopsis introns added

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. JME4472

   Plasmid

   #129658

   Purpose

   URA3ex_CrisprCas9-yl_RFP: CAS9 vector with URA3ex marker for gRNA cloning using GoldenGate

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSEED-mCherry

   Plasmid

   #219657

   Purpose

   Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGT10446

   Plasmid

   #192353

   Purpose

   L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-t35S_CGCT
   

   Expression

   Plant

   Available Since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED-Dendra2

   Plasmid

   #219645

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Dendra2 SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSEED-OLLAS:HA

    Plasmid

    #219647

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    OLLAS:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JME4390

    Plasmid

    #129656

    Purpose

    LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAGT5439

    Plasmid

    #192351

    Purpose

    L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tOCS_CGCT
    

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. JME4393

    Plasmid

    #129657

    Purpose

    LYS5ex_CrisprCas9-yl_RFP: CAS9 vector with LYS5ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEED-Moontag:HA

    Plasmid

    #219648

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    MoonTag:HA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSEED-EYFP:HA

    Plasmid

    #219656

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    EYFP:HA SEED Cassette
    

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JME5000

    Plasmid

    #129661

    Purpose

    EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEED-LoxP-sfGFP:ALFA

    Plasmid

    #219660

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette (LoxP)
    

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Level 1 P2 OsUbiP:Cas9:NosT

    Plasmid

    #165424

    Purpose

    GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Ubiquitin promoter :: Cas9 :: NosTerminator
    

    Use

    Synthetic Biology

    Promoter

    Os Ubiquitin promoter :: Cas9 :: NosTerminator

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEED-mScarlet3:ALFA

    Plasmid

    #219641

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mScarlet:ALFA SEED Cassette
    

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZmSWEET13a:ZmSWEET13a:GUS

    Plasmid

    #159535

    Purpose

    expresses ß--glucuronidase in cells expressing ZmSWEET13a

    Depositor

    Wolf Frommer

    Article

    Bezrutczyk et al Plant Cell. 2021 May 5;33(3):531-547. do

    Insert

    ZmSWEET13a
    

    Tags

    GUS

    Expression

    Bacterial and Plant

    Promoter

    native SWEET13a promoter

    Available Since

    July 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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