We narrowed to 12,680 results for: sequence

1. pcDNA3.2 mir-1-1 reporter hsa-mir-122

   Plasmid

   #46673

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-122 (MIR122 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. SRSF9_2xRRM_pGEX

   Plasmid

   #135120

   Purpose

   Recombinant expression of SRSF9 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   SRSF9 (SRSF9 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. prk5-MFSD12-TEV-3xFLAG-3xHA

   Plasmid

   #149504

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. SERPINA-bio-His

   Plasmid

   #52182

   Purpose

   Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINA (SERPINA1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SRSF8_RRM_pGEX

   Plasmid

   #135119

   Purpose

   Recombinant expression of SRSF8 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   SRSF8 (SRSF8 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGZ173 (pFA6a-SL-MNase-kanMX6)

   Plasmid

   #70234

   Purpose

   ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

   Depositor

   Steven Henikoff

   Article

   Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

   Insert

   Short linker and aa 83-231 of Mnase
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG-TurboID-GFP-PTS1

   Plasmid

   #209406

   Purpose

   Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-PTS1
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. evoCas9

   Plasmid

   #138558

   Purpose

   Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   evoCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, R661Q

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-dSpCas9

   Plasmid

   #92113

   Purpose

   Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Cbh

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. A2M-bio-His

    Plasmid

    #53423

    Purpose

    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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