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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 22,264 results for: fus

Showing: 5701 - 5730 of 22264 results

  3. pCOA-Leu-FvPPT1-fsr1

    Plasmid
    #179868
    Purpose
    Constitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1
    Depositor
    Jens Laurids Sørensen
    Article
    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)
    Inserts
    PPT1
    fsr1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Codon optimized for expression in Saccharomyces c…
    Promoter
    Yarrowia lipolytica EXP1 and Yarrowia lipolytica …
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    179868-plasmid-map-sequence-id-352936

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  4. pMCRW1

    Plasmid
    #122462
    Purpose
    Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
    Depositor
    Richard C. Willson
    Article
    Kourentzi et al Sci Rep. 2020 Mar 19;10(1):5078. doi: 10
    Insert
    NPM-ALK fusion protein (ALK, NPM1 Human)
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac promoter
    Available since
    May 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    122462-plasmid-map-sequence-id-243544

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  5. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

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  13. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid
    #169530
    Purpose
    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169530-plasmid-map-sequence-id-366213

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  14. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid
    #169529
    Purpose
    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169529-plasmid-map-sequence-id-364611

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  16. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid
    #169524
    Purpose
    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169524-plasmid-map-sequence-id-364607

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  18. pclbw-eGFPomp25

    Plasmid
    #58424
    Purpose
    Mammalian expression of GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    eGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    CMV/b-actin
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pclbw-PAGFPomp25

    Plasmid
    #58427
    Purpose
    Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane
    Depositor
    David Chan
    Article
    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi
    Insert
    paGFP-omp25 (Synj2bp Mouse)
    Use
    Retroviral
    Tags
    PAGFP
    Expression
    Mammalian
    Mutation
    contains aa's 109-145 of mouse omp25
    Promoter
    cmv-bactin
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  24. pDual

    Plasmid
    #207883
    Purpose
    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.
    Depositor
    David Edgell
    Article
    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206
    Inserts
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in th…
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207883-plasmid-map-sequence-id-422555

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  25. pCS2+mNeonGreen-C Cloning Vector

    Plasmid
    #128144
    Purpose
    To express a protein of interest fused to the C-terminus of mNeonGreen
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mNeonGreen fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128144-plasmid-map-sequence-id-248605

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  26. pCS2+mScarlet-C Cloning Vector

    Plasmid
    #128147
    Purpose
    To express a protein of interest fused to the C-terminus of mScarlet
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    mScarlet fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128147-plasmid-map-sequence-id-248619

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  27. pZP06B

    Plasmid
    #232321
    Purpose
    Make sacB a counterselection marker to replace galK
    Depositor
    Chenggang Wu
    Insert
    PrpsJ-sacB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232321-plasmid-map-sequence-id-462085

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  28. pBCG10

    Plasmid
    #232323
    Purpose
    Make mazF a counterselection marker to replace sacB in pZP06C
    Depositor
    Chenggang Wu
    Insert
    mazF-tetR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232323-plasmid-map-sequence-id-462282

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  29. pcDNA3.1-hSyncytin-1(deltaFP)

    Plasmid
    #182443
    Purpose
    hSyncytin-1, fusion peptide deletion(320-340)
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    no fusion peptide (320-340)
    Promoter
    CMV-F
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182443-plasmid-map-sequence-id-357706

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Showing: 5701 - 5730 of 22264 results