Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 116,162 results for: mpi

Showing: 9501 - 9550 of 116162 results

  1. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

    Enlarge

  5. pSBbi-Pur-LPAR

    Plasmid
    #125205
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide
    Depositor
    Eric Davis
    Insert
    Lyn-Phospho-Activity-Reporter, Empty
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125205-plasmid-map-sequence-id-243607

    Enlarge

  6. pcDNA-mTRAF3ip3∆TM-CFP

    Plasmid
    #125182
    Purpose
    Expresses mouse TRAF3IP3 ∆TM in mammalian cells
    Depositor
    Shao-Cong Sun
    Insert
    TRAF3ip3 (Traf3ip3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    K87R and S359P- See depositor comments below
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    125182-plasmid-map-sequence-id-243310

    Enlarge

  7. pZSCAN18-donor

    Plasmid
    #113821
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP
    Depositor
    Kevin White
    Insert
    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113821-plasmid-map-sequence-id-241248

    Enlarge

  10. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

    Enlarge

  12. pZNF561-donor

    Plasmid
    #113801
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP
    Depositor
    Kevin White
    Insert
    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113801-plasmid-map-sequence-id-229885

    Enlarge

  13. pZNF700-donor

    Plasmid
    #112385
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF700 with GFP
    Depositor
    Kevin White
    Insert
    ZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112385-plasmid-map-sequence-id-229845

    Enlarge

  14. pZNF169-donor

    Plasmid
    #112376
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF169 with GFP
    Depositor
    Kevin White
    Insert
    ZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112376-plasmid-map-sequence-id-229740

    Enlarge

  15. pZFP1-donor

    Plasmid
    #112374
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFP1 with GFP
    Depositor
    Kevin White
    Insert
    ZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112374-plasmid-map-sequence-id-229736

    Enlarge

  16. pZNF227-donor

    Plasmid
    #112368
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF227 with GFP
    Depositor
    Kevin White
    Insert
    ZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112368-plasmid-map-sequence-id-229716

    Enlarge

  17. pOTX1-donor

    Plasmid
    #112364
    Purpose
    CRISPR donor plasmid to tag human transcription factor OTX1 with GFP
    Depositor
    Kevin White
    Insert
    OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112364-plasmid-map-sequence-id-229691

    Enlarge

  18. pZNF175-donor

    Plasmid
    #112347
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP
    Depositor
    Kevin White
    Insert
    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112347-plasmid-map-sequence-id-229660

    Enlarge

  19. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  21. pZNF354C-donor

    Plasmid
    #112339
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354C with GFP
    Depositor
    Kevin White
    Insert
    ZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112339-plasmid-map-sequence-id-228576

    Enlarge

  22. pZNF347-donor

    Plasmid
    #112326
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF347 with GFP
    Depositor
    Kevin White
    Insert
    ZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112326-plasmid-map-sequence-id-228548

    Enlarge

  23. pHH0103 HYPB WW domain

    Plasmid
    #104267
    Purpose
    Bacterial expression of WW domain from HYPB
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    HYPB WW domain (SETD2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104267-plasmid-map-sequence-id-203491

    Enlarge

  24. pTU1-D-spsCD

    Plasmid
    #112788
    Purpose
    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112788-plasmid-map-sequence-id-218383

    Enlarge

  25. pTU1-A-spsCU

    Plasmid
    #112787
    Purpose
    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    spsCU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112787-plasmid-map-sequence-id-218381

    Enlarge

  27. pTU1-C-cat

    Plasmid
    #112791
    Purpose
    Chloramphenicol resistance cassette (expressing chloramphenicol acetyltransferase)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    cat
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112791-plasmid-map-sequence-id-218393

    Enlarge

  28. pTU1-D-rrnOD

    Plasmid
    #112786
    Purpose
    Downstream homology arm, rrnO-23S (23S rRNA)
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    rrnOD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112786-plasmid-map-sequence-id-218378

    Enlarge

  29. pTU1-D-amyED

    Plasmid
    #112782
    Purpose
    Downstream homology arm, amyE encodes _-amylase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    amyED
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112782-plasmid-map-sequence-id-218373

    Enlarge

  30. pTU1-A-amyEU

    Plasmid
    #112781
    Purpose
    Upstream homology arm, amyE encodes _-amylase
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    amyEU
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112781-plasmid-map-sequence-id-218371

    Enlarge

  32. pHH0103 WWOX WW domain #2

    Plasmid
    #104236
    Purpose
    Bacterial expression of WW domain #2 from WWOX
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    WWOX WW domain #2 (WWOX Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104236-plasmid-map-sequence-id-204371

    Enlarge

  33. DsRed-Nix-S212A

    Plasmid
    #100762
    Purpose
    Mammalian expression of Fluorescent Nix S212A
    Depositor
    Joseph Gordon
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    S212A
    Promoter
    CMV
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100762-plasmid-map-sequence-id-193062

    Enlarge

  34. pAM-35s-CIPK5-YFPn-1

    Plasmid
    #102392
    Purpose
    split YFP. Plant expression of CIPK5-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    CIPK5 (CIPK5 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102392-plasmid-map-sequence-id-196617

    Enlarge

  35. pAM-35s-Snrk2.3-YFPn-1

    Plasmid
    #102391
    Purpose
    split YFP. Plant expression of Snrk2.3-YFPn-1
    Depositor
    Silke Robatzek
    Insert
    SNRK2.3 (SNRK2.3 Mustard Weed)
    Use
    Tags
    YFPn
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102391-plasmid-map-sequence-id-196616

    Enlarge

  36. pAM-35s-CIPK5-YFPc-1

    Plasmid
    #102387
    Purpose
    split YFP. Plant expression of CIPK5-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CIPK5 (CIPK5 Mustard Weed)
    Use
    Tags
    YFPc
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    102387-plasmid-map-sequence-id-195327

    Enlarge

  37. DsRed-Nix-S212D

    Plasmid
    #100764
    Purpose
    Mammalian expression of Fluorescent Nix S212D
    Depositor
    Joseph Gordon
    Insert
    dsRed-Nix-S212D (BNIP3L Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    S212D
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100764-plasmid-map-sequence-id-193067

    Enlarge

  38. pGAD424-hL1

    Plasmid
    #89423
    Purpose
    Two-hybrid system
    Depositor
    Fritz Rathjen
    Insert
    L1CAM (L1CAM Human)
    Use
    Tags
    Gal4 AD
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    89423-plasmid-map-sequence-id-177566

    Enlarge

  41. 5J3U (TgPKA, 583.m05422)

    Plasmid
    #79525
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    583.m05422
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79525-plasmid-map-sequence-id-150111

    Enlarge

  42. 5FEA (LdBRD19, LdBPK_363520.1)

    Plasmid
    #79520
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363520.1
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79520-plasmid-map-sequence-id-150104

    Enlarge

  43. 5CZG (Tb427.10.7420)

    Plasmid
    #79517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427.10.7420
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    June 15, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79517-plasmid-map-sequence-id-150098

    Enlarge

  44. Plekhm1 C279 pGEX 4T2-GST

    Plasmid
    #73835
    Purpose
    Express human Plekhm1 (C279 to end domain) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Use
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned last 279 aa from human Plek…
    Promoter
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. Plekhm1 -PH1/2 pGEX 4T2-GST

    Plasmid
    #73831
    Purpose
    Express human Plekhm1 (PH1 and PH2 domains) in bacterial cells
    Depositor
    Paul Odgren
    Insert
    Plekhm1 (PLEKHM1 Human)
    Use
    Tags
    Gst
    Expression
    Bacterial
    Mutation
    to vector was cloned Ph1 and Ph2 domains of human…
    Promoter
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  46. pJK611

    Plasmid
    #72532
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72532-plasmid-map-sequence-id-132620

    Enlarge

  47. pJK626

    Plasmid
    #72461
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Mutation changes serine-57 to cysteine, tyrosine-…
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72461-plasmid-map-sequence-id-133899

    Enlarge

  48. pJK292

    Plasmid
    #73371
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to asparagine and histidine-…
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73371-plasmid-map-sequence-id-135107

    Enlarge

  49. pJK293

    Plasmid
    #73372
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to phenylalanine and histidi…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73372-plasmid-map-sequence-id-135108

    Enlarge

  50. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

    Enlarge

Showing: 9501 - 9550 of 116162 results