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We narrowed to 92 results for: mpi

Showing: 1 - 20 of 92 results

  3. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...regions of live zebrafish larvae expressing FRCaMPi or SomaFRCaMPi in neurons; scale bars 50 µm. Reproduced...transfer tracker Red calcium indicators PinkyCaMP and FRCaMPi Micropeptide killswitch for biomolecular condensates....0510511103      Red calcium indicators PinkyCaMP and FRCaMPi By Mike Lacy Many popular genetically encoded calcium...Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from the Kiryl Piatkevich Lab (Zhou, Zhu, Eom, ...alongside green fluorescent and optogenetic tools. FRCaMPi uses an inverted topology of FRCaMP (itself based... similar in brightness to jRGECO1a and FRCaMP, FRCaMPi had better dynamic range and sensitivity, in part...resting neurons. Their soma-targeted variant SomaFRCaMPi also performed substantially better than other...

  5. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria..., it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is just the ...the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and...curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed...

  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...Carbenicillin is more stable than Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics...protein synthesis Bactericidal 25-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal...most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast and plates should...

  8. Open Enzyme Collection

    Type
    Collection
    ...marker for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols...

  9. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L HSPA1L GFP CMV Parkinson's Harm Kampinga 19511 pcDNA5...Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L HSPA1L His CMV Parkinson's Harm Kampinga 20030 pSB819...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...issue. The genes and their antibiotics   Ampicillin Ampicillin, often just called amp, is the historical... with most bacteria? No satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name...

  12. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...from one location in the genome to another. This jumping is what caught the eye of Barabara McClinntock ...138920210790886871 Pray, L. (2008) Transposons: The jumping genes. Nature Education 1(1):204. https://www.nature.com....nature.com/scitable/topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger...

  14. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  18. Finding Your Science Policy Path

    Type
    Blog Post
    ...it so wonderful. The most challenging part of jumping into this career path from academia is the uncertainty...science policy roles exist. Explore these resources compiled by the AAAS Science and Technology Policy Fellowship...

  19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ..., the optimal viral titer must be determined empirically (this is the case for any viral injection into...reach the axon terminal. Again, this must be empirically determined in your own system. Once you are confident...
Showing: 1 - 20 of 92 results