We narrowed to 92 results for: mpi

1. Visualizing Protein Turnover In Situ

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   Blog Post

   ...privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments...

2. Immunology Research Plasmids and Resources

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   ... MIP-3, MIP3, MPIF-1, SCYA23 CCL24 chemokine (C-C motif) ligand 24 Ckb-6, MPIF-2, MPIF2, SCYA24 CCL25 ...

3. Six Spooky Science Stories and Halloween at Addgene

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   ...next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria..., it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is just the ...the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and...curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed...

4. Plasmids 101: Antibiotic Resistance Genes

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   ...Carbenicillin is more stable than Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics...protein synthesis Bactericidal 25-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal...most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast and plates should...

5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

6. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   ...issue. The genes and their antibiotics   Ampicillin Ampicillin, often just called amp, is the historical... with most bacteria? No satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name...

7. Plasmids 101: Using Transposons in the Lab

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   ...from one location in the genome to another. This jumping is what caught the eye of Barabara McClinntock ...138920210790886871 Pray, L. (2008) Transposons: The jumping genes. Nature Education 1(1):204. https://www.nature.com....nature.com/scitable/topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger...

8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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   Blog Post

   ...the other hand some software tools make use of empirically derived and/or prior knowledge to inform on the... Hsu et al., 2013) used a combination of both empirical and approximate methods. The details of their ...“high mismatch limit”, “approximate design”, “empirical design”, “single-PAM design”, and “multi-PAM design...

9. Neurodegeneration Plasmid Collection

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   ...Parkinson's Harm Kampinga 19484 pcDNA5/FRT/TO GFP HSPA1L HSPA1L GFP CMV Parkinson's Harm Kampinga 19511 pcDNA5...Parkinson's Harm Kampinga 19538 pcDNA5/FRT/TO HIS HSPA1L HSPA1L His CMV Parkinson's Harm Kampinga 20030 pSB819...

10. Synthetic Biology - Assembly Standards Guide

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    ...when creating a new part, with an additional 5' clamping sequence such as GTTTCTTC). Note that all prefix...

11. AAVED

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    ...For All Attendees: To help us best prepare and compile resources for this meeting's publication, we are...

12. CRISPR Plasmids - Bacteria

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    ...44251 pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have suggestions...

13. Save Time and Money by Making Your Own Competent Cells

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    Blog Post

    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

14. Open Enzyme Collection

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    ...marker for all plasmids in this collection is ampicillin. Find more information on the enzymes and protocols...

15. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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    ... from humans and other species. By accurately compiling genome-wide datasets, which are often obscured... data are also available via comprehensive pre-compiled downloads and via a full-featured REST Web Service...

16. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...constructing appropriate controls may requires that you empirically test a number of mutants to identify those that...

17. Finding Your Science Policy Path

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    Blog Post

    ...it so wonderful. The most challenging part of jumping into this career path from academia is the uncertainty...science policy roles exist. Explore these resources compiled by the AAAS Science and Technology Policy Fellowship...

18. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ..., the optimal viral titer must be determined empirically (this is the case for any viral injection into...reach the axon terminal. Again, this must be empirically determined in your own system. Once you are confident...

19. Genomic Deletions in Mammalian Cell Lines

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    ...lysogeny broth (LB) agar plate with 100 μg/ml ampicillin and incubate O/N at 37 °C. Pick 2 - 3 colonies...

20. Validated gRNA Sequences

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    ...for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database as well as...