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We narrowed to 71 results for: mpi

Showing: 1 - 20 of 71 results

  1. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform similar experiments...

  2. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...next unsuspecting ant to stumble upon. Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria..., it sucks out its contents (hence the name Vampirovibrio). What’s left of the algae cell is just the ...the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in culture alone and...curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." PeerJ 3 (2015): e968. PubMed...

  3. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...Carbenicillin is more stable than Ampicillin and can be used in place of Ampicillin in most applications. Antibiotics...protein synthesis Bactericidal 25-100 ug/mL Ampicillin beta-lactam Inhibits cell wall synthesis Bactericidal...most antibiotics viable for at least 6 months. Ampicillin breaks down especially fast and plates should...

  4. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...issue. The genes and their antibiotics   Ampicillin Ampicillin, often just called amp, is the historical... with most bacteria? No satellite colonies? Ampicillin Fair {% icon icon_set="fontawesome-5.14.0" name...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...from one location in the genome to another. This jumping is what caught the eye of Barabara McClinntock ...138920210790886871 Pray, L. (2008) Transposons: The jumping genes. Nature Education 1(1):204. https://www.nature.com....nature.com/scitable/topicpage/transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger...

  7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...the other hand some software tools make use of empirically derived and/or prior knowledge to inform on the... Hsu et al., 2013) used a combination of both empirical and approximate methods. The details of their ...“high mismatch limit”, “approximate design”, “empirical design”, “single-PAM design”, and “multi-PAM design...

  8. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...it so wonderful. The most challenging part of jumping into this career path from academia is the uncertainty...science policy roles exist. Explore these resources compiled by the AAAS Science and Technology Policy Fellowship...

  12. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ..., the optimal viral titer must be determined empirically (this is the case for any viral injection into...reach the axon terminal. Again, this must be empirically determined in your own system. Once you are confident...

  13. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...are of interest to you, our readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our ...

  16. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... RNA Extraction Without A Kit blog post, we've compiled five methods for extracting DNA without a kit ...

  18. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ... 2)      In June, the White House honored 13 “Champions of Change,” including our recent guest Drew Endy...
Showing: 1 - 20 of 71 results