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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Publication

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ABCG1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176944

Purpose

Mammalian expression vector encoding ABCG1 and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]

Plasmid

#78174

Purpose

AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-EGFP

Plasmid

#177136

Purpose

Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

Depositor

Robert Sobol

Publication

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

EGFP

Expression

Bacterial

Promoter

Tac

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-EYFP

Plasmid

#35519

Depositor

Karl Deisseroth

Publication

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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C17A

Plasmid

#27801

Depositor

Steven Vogel

Publication

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-17-Amber

Use

Fret donor control

Tags

Amber and Cerulean

Expression

Mammalian

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-MAPK15

Plasmid

#23697

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK15 (MAPK15 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

Plasmid

#89693

Purpose

Luciferase reporter plasmid targeted at csr-1 locus

Depositor

Christian Hong

Publication

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Insert

gsy-1 promoter, luciferase

Use

Yeast episomal vector

Tags

luciferase

Promoter

gsy-1

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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HOXC4 (human) MSCV

Plasmid

#8546

Depositor

Corey Largman

Publication

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXC4 (HOXC4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains improved ribosomal binding site and NcoI…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pHL-EF1a-SphcCas9(D10A)-iP-A

Plasmid

#60600

Purpose

Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Publication

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

CRISPR Cas9 D10A
puromycin resistance gene

Use

CRISPR

Expression

Mammalian

Mutation

Changed Asp 10 to Ala, Codon usage optimized for …

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Publication

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Expression

Bacterial

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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FLS-PC1

Plasmid

#41520

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Polycystin-1 (PKD1 Human)

Tags

Myc/His

Expression

Mammalian

Mutation

sequence from 4107 - 4303 of PKD1

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-ALPK2

Plasmid

#23493

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALPK2 (ALPK2 Human)

Use

Gateway Cloning

Mutation

A117G

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MKNK1

Plasmid

#23531

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK1 (MKNK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NME4

Plasmid

#23584

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME4 (NME4 Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-YAP1

Plasmid

#79503

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

FLAG

Promoter

no Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sgRNA1_ASCL1

Plasmid

#64129

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-BCKDK

Plasmid

#23794

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BCKDK (BCKDK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles