We narrowed to 7,164 results for: 11

1. GAL-p300-501-999

   Plasmid

   #89100

   Purpose

   mammalian expression of human p300 amino acids 501-999 with N-terminal GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

   Insert

   p300 (EP300 Human)
   

   Use

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 501-999

   Promoter

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1.Gα13-LgB106

   Plasmid

   #134364

   Purpose

   Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα13-LgB106 (GNA13 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB1-A11(2-88)-Strep

   Plasmid

   #196214

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)

   Plasmid

   #138555

   Purpose

   Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ADAMTS5_pCSdest

   Plasmid

   #53819

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   ADAMTS5 (ADAMTS5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pQFlag-USP7 WT puroR

   Plasmid

   #46751

   Purpose

   Retroviral vector that expresses wild type Flag-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS_cAMPFIRE-H

   Plasmid

   #182285

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-H under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-H (cAMP sensor)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMma PylT_FLAG-PcKRS

   Plasmid

   #140022

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   PcKRS (pylS Methanosarcina bakeri)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   M241F, A267S, Y271C, L274M

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM19

    Plasmid

    #123655

    Purpose

    Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pIEx

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NG

    Plasmid

    #138566

    Purpose

    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    SpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAL-p300-1000-1600

    Plasmid

    #89101

    Purpose

    mammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1000-1600

    Promoter

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GAL-p300-2-300

    Plasmid

    #89098

    Purpose

    mammalian expression of human p300 amino acids 2-300 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 2-300

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GAL-p300-1901-2414

    Plasmid

    #89103

    Purpose

    mammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300 (EP300 Human)
    

    Use

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Mutation

    aa 1901-2414

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-PM-TORCAR

    Plasmid

    #64930

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    Lyn-TORCAR (EIF4EBP1 Human, Synthetic)
    

    Use

    Tags

    Cerulean, N-terminal targeting sequence from Lyn …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_cAMPFIRE-L-R279E

    Plasmid

    #182286

    Purpose

    Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HypaCas9

    Plasmid

    #138557

    Purpose

    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    HypaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    N692A, M694A, Q695A, H698A

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3_cAMPFIRE-L-R279E

    Plasmid

    #182282

    Purpose

    Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV_TRET_hNgn1_UBC_Bla

    Plasmid

    #61473

    Purpose

    3rd generation lentiviral vector; TetON promoter driving human Ngn1; constitutive expression of Blasticidine selection marker

    Depositor

    Ron Weiss

    Article

    Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

    Inserts

    Ngn1 (NEUROG1 Human)
    Bla
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-EMTB-TurboID-V5

    Plasmid

    #190736

    Purpose

    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Tags

    Myc, TurboID, and V5

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    CMV

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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