We narrowed to 4,337 results for: Pet2

1. Dom34 Y300A

   Plasmid

   #19110

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   Y300A

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

2. Dom34 K45A

   Plasmid

   #19111

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   K45A

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

3. p40deltaPX (w/out aa1-135)

   Plasmid

   #19019

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox (NCF4 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa136-148 (PX domain, truncated)

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pCpSRb-5

   Plasmid

   #13727

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   SRβ circularly permuted D210E188 improved solubil…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDrSR beta deltaN

   Plasmid

   #13730

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   expresses residues 60-266 of Danio rerio SRβ with…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT22-GluCNZ-6His

   Plasmid

   #213504

   Purpose

   Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.

   Depositor

   John Denu

   Article

   Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12

   Insert

   Glucanase (glucan endo-1,3-beta-glucosidase)
   

   Tags

   6His tag

   Expression

   Bacterial

   Mutation

   1-36 Signal Sequence Deletion

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-NLS-Cas9-6xHis

   Plasmid

   #62934

   Purpose

   Expression of NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   NLS-Cas9-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-NbALFA

   Plasmid

   #159987

   Purpose

   For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mCherry-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mCherry and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIIS793

    Plasmid

    #238206

    Purpose

    Expresses TaTasR with an N-terminal Twin-Strep-SUMO tag. KanR

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    TaTasR
    

    Tags

    TwinStrepII-bdSUMO

    Expression

    Bacterial

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBJG1 T7-8xHis-OGT

    Plasmid

    #154271

    Purpose

    Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

    Depositor

    Suzanne Walker

    Article

    Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Tags

    8x His, HRV3C protease site, and T7 leader

    Expression

    Bacterial

    Mutation

    bacterial codon optimized

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. I48 ELP

    Plasmid

    #68937

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

    Depositor

    Andrew MacKay

    Article

    Shi et al J Control Release. 2013 Nov 10;171(3):33

    Insert

    I48 ELP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-28a-YTH domain of YTHDF2

    Plasmid

    #228776

    Purpose

    Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

    Depositor

    Huiqing Zhou

    Article

    Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

    Insert

    YTH domain of YTHDF2 (YTHDF2 Human)
    

    Tags

    6*His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-21a(+)-AK1-His

    Plasmid

    #118977

    Purpose

    Bacterial expression plasmid for Adenylate kinase isoenzyme 1

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    adenylate kinase isoenzyme 1 AK1 (AK1 Chicken)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pBEL2319

    Plasmid

    #195697

    Purpose

    Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    Foldon-MlaC
    

    Expression

    Bacterial

    Mutation

    6xHis-Foldon-(Gly-Ser-Linker)-MlaC

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AviTag-SpyTag003-MBP

    Plasmid

    #184227

    Purpose

    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

    Depositor

    Mark Howarth

    Article

    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

    Insert

    AviTag-SpyTag003-MBP
    

    Tags

    AviTag, His6, SpyTag003, and Thrombin cleavage si…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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