We narrowed to 6,627 results for: hwa

1. lentiCRISPR v2-sgSLC7A11/xCT-2

   Plasmid

   #161819

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. U6-sgfiller-eCas9-T2A-BlastR

   Plasmid

   #172437

   Purpose

   Expresses eCas9, BlastR and sgRNA cloning site

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Inserts

   eCas9
   BlastR
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDC40

   Plasmid

   #155444

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CDC40 (CDC40 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PABPC1

   Plasmid

   #155805

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PABPC1 (PABPC1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. ALDH18A1

   Plasmid

   #155402

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPC1-hVAP-B

   Plasmid

   #104448

   Purpose

   Expression of human VAP-B fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Tags

   EGFP and HA

   Expression

   Mammalian

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDF RBP-FL P53

   Plasmid

   #174042

   Purpose

   Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

   Depositor

   Stewart Loh

   Article

   Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

   Insert

   P53 gene (TP53 Human)
   

   Tags

   Thermoanaerobacter Tencongenesis ribose binding p…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPI4-Strep-SNAP-CD28-GCN4-His6

   Plasmid

   #223585

   Purpose

   Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP-proα2(I)

   Plasmid

   #119826

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with GFP between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GLE1

    Plasmid

    #155625

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GLE1 (GLE1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGF-GAS4X

    Plasmid

    #225751

    Purpose

    This plasmid was designed to selectively monitor the activation of the STAT1 signaling pathway in NK cells, a pathway known to play a crucial role in regulating NK cells' activation.

    Depositor

    Eun Hee Han

    Article

    Min et al Commun Biol. 2024 Sep 30;7(1):1222. doi:

    Insert

    interferon gamma activating sequence (GAS) (STAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-15b_LC3B

    Plasmid

    #73949

    Purpose

    For recombinant expression of human LC3B/MAP1LC3B in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    LC3B (MAP1LC3B Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. eGFP-proα1(I)

    Plasmid

    #119843

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid

    #196182

    Purpose

    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Inserts

    GSK3B (GSK3B Human)
    λPPase
    

    Tags

    GST, HA, His6, and MBP

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT/TO/HIS/FLAG/HA-HNRNPD

    Plasmid

    #38066

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    HNRNPD heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) (HNRNPD Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSecTaq2-hGas6-Myc-6xHis

    Plasmid

    #226523

    Purpose

    Expresses TAM Receptor Ligand human GAS6

    Depositor

    Raymond Birge, Sergei Kotenko

    Article

    Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

    Insert

    Human GAS6 (GAS6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. eGFP-proα2(I)-L

    Plasmid

    #120160

    Purpose

    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α2 chain (Col1a2 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Col1a2 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid

    #182424

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    

    Tags

    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-REST_sh_seq1_WPRE

    Plasmid

    #127573

    Purpose

    shRNA to knock down RE1-silencing transcription factor (REST)

    Depositor

    Malin Parmar

    Article

    Drouin-Ouellet et al EMBO Mol Med. 2017 Aug;9(8):1117-1131. d

    Insert

    shRNA for knock down of REST (REST Human)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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