We narrowed to 20,245 results for: ACE;

1. I13453 (pBAD)_FB

   Plasmid

   #66018

   Purpose

   MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Controllable promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. I13453 (pBAD)_GB

   Plasmid

   #66019

   Purpose

   MoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Controllable promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. B0033m_BC

   Plasmid

   #66021

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. B0034m_BC

   Plasmid

   #66022

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C0080 (araC)_CD

   Plasmid

   #66029

   Purpose

   MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   Transcription factor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pES006 (pTet-qacR 2/pQacA-deGFP)

   Plasmid

   #69035

   Purpose

   reporter with qacR 2 on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…

   Promoter

   pQacA and pTet

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pES007 (pTet-qacR 5/pQacA-deGFP)

   Plasmid

   #69036

   Purpose

   reporter with qacR 5 on single plasmid

   Depositor

   Richard Murray

   Article

   de Los Santos et al ACS Synth Biol. 2015 Aug 19.

   Inserts

   qacR (qacR Synthetic)
   deGFP
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   W61Y, E90Q, F102S, M116Q, Y119L, E120Q

   Promoter

   pQacA and pTet

   Available Since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. NET33 pEGFP-N2 (594)

   Plasmid

   #61987

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET33 (SCARA5 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pSG5 N-RFP SADBs

   Plasmid

   #67153

   Purpose

   pSG5 containing N-terminally RFP tagged mouse SADBs

   Depositor

   Ana Carrera, Maria Alvarado-Kristensson

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   Brsk1 (Brsk1 Mouse)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSG5 N-HA SADBs

    Plasmid

    #67149

    Purpose

    pSG5 containing N terminally HA tagged mouse SADBs

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    Brsk1 (Brsk1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SV40, T7

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pZS1[Iq:LacI-T]

    Plasmid

    #60756

    Purpose

    Contains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Insert

    LacI-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI without the tetramerization domain and the T…

    Promoter

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET12a-AL-103 Y32F Y96F

    Plasmid

    #47092

    Depositor

    Marina Ramirez-Alvarado

    Article

    DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

    Insert

    AL-103 Y32F Y96F (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Y32F Y96F

    Promoter

    T7

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pET12a-AL-09 Y32F Y96F

    Plasmid

    #47083

    Depositor

    Marina Ramirez-Alvarado

    Article

    DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

    Insert

    AL-09 Y32F Y96F (IGKV3-20 Human)
    

    Use

    Tags

    ompT

    Expression

    Bacterial

    Mutation

    Y32F Y96F

    Promoter

    T7

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pCDM4-GLY

    Plasmid

    #49808

    Purpose

    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Emerin pEGFP-C1 (637)

    Plasmid

    #61993

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    Emerin (EMD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cacnb2a (rat) in pMT2 vector

    Plasmid

    #107424

    Purpose

    Expresses Calcium channel beta2a auxillary subunit in mammalian cells

    Depositor

    Annette Dolphin

    Article

    Wyatt et al J Physiol. 1998 Jul 15;510 ( Pt 2):347-6

    Insert

    calcium channel beta2a auxillary subunit (Cacnb2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-DCX-TurboID-V5

    Plasmid

    #190740

    Purpose

    Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

    Depositor

    Jeremy Baskin

    Article

    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

    Insert

    doublecortin (DCX Human)
    

    Use

    Tags

    TurboID and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111923

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. plenti hSyn PKM1

    Plasmid

    #220920

    Purpose

    Neuronal specific expression of PKM1

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PKM1 (PKM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330-CETN2

    Plasmid

    #227291

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CETN2 (CETN2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LAMP1-RpHLuorin2

    Plasmid

    #171720

    Purpose

    Lysosomal expression of ratiometric pHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)
    

    Use

    Tags

    ratiometric pHluorin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-FLEx-ER-TurboID

    Plasmid

    #160857

    Purpose

    Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Has Service

    AAV8

    Insert

    ER TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-mem-TurboID

    Plasmid

    #149409

    Purpose

    Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    mem-TurboID
    

    Use

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p)

    Plasmid

    #70191

    Purpose

    Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137.

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    ClpX (clpX )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCK054 pDEST exorh:mCerulean

    Plasmid

    #195985

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge