We narrowed to 20,388 results for: ACE;

Showing: 5881 - 5910 of 20388 results

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPD0054_pIVTRup_mCherry2

Plasmid

#242545

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

mCherry

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR

Plasmid

#192446

Purpose

Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEx-ER-TurboID

Plasmid

#160857

Purpose

Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Has Service

AAV8

Insert

ER TurboID

Use

AAV

Tags

HA and V5

Promoter

CAG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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LAMP1-RpHLuorin2

Plasmid

#171720

Purpose

Lysosomal expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to LAMP1 (LAMP1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a S25N pcDNA3.1 Zeo (+)

Plasmid

#206080

Purpose

dominant-negative Rab11a mutant - S25N mutation causes inhibition of Rab11a-dependent recycling

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ExRai-AMPKAR(T/A)

Plasmid

#192447

Purpose

Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

Expression

Mammalian

Mutation

Thr 6 in AMPK substrate mutated to Ala.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.2

Plasmid

#218823

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1

Plasmid

#218817

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Bacterial

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1.1

Plasmid

#218818

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Bacterial

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-iLACCO1.2

Plasmid

#218819

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Expression

Bacterial

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-DiLACCO1

Plasmid

#218820

Purpose

Bacterial expression of genetically encoded green fluorescent L-lactate control biosensor

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

DiLACCO1

Expression

Bacterial

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

Plasmid

#207664

Purpose

Neuropeptide degradation

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

NEP (MME Human)

Use

AAV and Cre/Lox

Tags

mRUBY3

Mutation

Addition of signaling peptide (POMC) on the N-ter…

Promoter

hSynapsin

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pcDNA3

Plasmid

#206053

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_GCaMP6s

Plasmid

#164591

Purpose

Stable integration and expression of GCaMP6s in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

GCaMP6s

Use

Lentiviral

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

R-FlincA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCryptDel4.8

Plasmid

#141293

Purpose

Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Inserts

RelB
gRNA targetting pMUT2

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Native promoter from pMUT2 relB/relE operon and P…

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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SpRY Cas9

Plasmid

#188491

Purpose

Expresses FLAG tagged SpRY Cas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-cs1-PuroR-T2A-BFP

Plasmid

#230934

Purpose

gRNA cloning vector with direct capture sequence 1 in stem loop 2

Depositor

Charles Gersbach

Article

Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

TagBFP

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTnsABC

Plasmid

#160031

Purpose

Expresses V. cholerae TnsABC

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

VchTnsA, VchTnsB, VchTnsC

Use

CRISPR; Transposon

Expression

Bacterial

Mutation

no

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRXRα

Plasmid

#135910

Purpose

Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Human retinoid X receptor- alpha (RXRA Human)

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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