We narrowed to 27,269 results for: Tat

1. tagSrc(WT)-mCer3 S208F/V224L

   Plasmid

   #179138

   Purpose

   mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   Src, SsrA
   

   Use

   Tags

   mCerulean3 (S208F/V224L)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ELF5_pet28a

   Plasmid

   #131659

   Purpose

   Bacterial expression of ETS gene ELF5

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELF5 (ELF5 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO GFP HSPA14

   Plasmid

   #19488

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   HSPA14 (HSPA14 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. K15/LacZ

   Plasmid

   #44265

   Depositor

   George Cotsarelis

   Article

   Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8

   Insert

   K15 promoter (-4.8) (Krt15 Mouse)
   

   Use

   Mouse Targeting; Β-galactosidase

   Tags

   LacZ

   Expression

   Mammalian

   Mutation

   Contains the murine K15 promoter (-4.8) fragment

   Promoter

   murine K15 promoter (-4.8) fragment

   Available since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGFP-PTEN C124A

   Plasmid

   #50520

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C124A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pIRESpuro2-UBXD1-mCherry

   Plasmid

   #31835

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   UBXD1 (UBXN6 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pETDuet1-WT-LanCL2-C-Ter-His

   Plasmid

   #154186

   Purpose

   To express LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p164-pAAV-mDlx-FLEX-mScarlet

   Plasmid

   #204689

   Purpose

   Fluorescent reporter under the control of the Dlx promotor

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   mScarlet palmitoylation

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMyc1ElbLuc,

   Plasmid

   #53243

   Purpose

   reporter plasmid containing 1 myc cDNA binding sites

   Depositor

   Roger Davis

   Article

   Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

   Insert

   pMyc1ElbLuc (MYC Human)
   

   Use

   Tags

   Expression

   Mutation

   To prepare plasmids with one, two, three, and fou…

   Promoter

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pFSW Doc2alpha WT

    Plasmid

    #128812

    Purpose

    To make virus expressing Doc2alpha WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2alpha (Doc2a Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFSW Doc2beta WT

    Plasmid

    #128814

    Purpose

    To make virus expressing Doc2beta WT

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PSMD14 E6.4 gRNA

    Plasmid

    #90859

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PSMD14

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PSMD14 (Guide Designation E6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO GFP DNAJA4

    Plasmid

    #19494

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA4 (DNAJA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47454

    Purpose

    epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-SID4X
    

    Use

    AAV

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-SNAI2

    Plasmid

    #170702

    Purpose

    doxycycline-inducible overexpression of SNAI2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SNAI2 (SNAI2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-sgAtm/Cre

    Plasmid

    #89643

    Purpose

    Expresses an Atm-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Atm (Atm Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pD765 FDFT1 3'UTR

    Plasmid

    #105765

    Purpose

    Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

    Depositor

    Joseph Ziegelbauer

    Article

    Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

    Insert

    FDFT1 3'UTR (FDFT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5FRT/TO-DVC1-Strep-HA

    Plasmid

    #113481

    Purpose

    Expression of human DVC1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    DVC1 (SPRTN Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-SETDB1_1

    Plasmid

    #36347

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    SETDB1 (SETDB1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

21. MSCV Puro SCRIB P305L

    Plasmid

    #88887

    Purpose

    SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Proline to Leucine mutation @ 305th amino acid

    Promoter

    MSCV promoter

    Available since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Hy_pMT Flag-Hel25E WT

    Plasmid

    #110123

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pWZL Blast H-Ras G12V T35S

    Plasmid

    #12278

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V T35S (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The T35S mutat…

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

24. p4979 pcDNA4-E6AP III L502P

    Plasmid

    #32892

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    L502P

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

25. p4978 pcDNA4-E6AP III E550L

    Plasmid

    #32891

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    E550L

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pSLIK-Venus-TmiR-Luc

    Plasmid

    #25739

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBT3019

    Plasmid

    #122541

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::SKL
    

    Use

    Tags

    SKL

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGST TCF4 DBD

    Plasmid

    #16488

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    TCF4 DNA binding domain (TCF7L2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 265-496

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pGEMHE-POLArISact

    Plasmid

    #164970

    Purpose

    T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCR8/GW/TOPO-SRF

    Plasmid

    #98618

    Purpose

    Gateway entry TOPO TA cloning vector for SRF

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    SRF (Srf Mouse)
    

    Use

    Topo ta cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRRL-VinculinVenus-S1033A

    Plasmid

    #211896

    Purpose

    Vinculin (chicken) with S1033 unphosphorylatable point mutation (S1033A) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinVenus-S1033A (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    VenusA206K

    Expression

    Mutation

    mutated vinculin serine 1033 to alanine (S1033A),…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRRL-VinculinVenus-S1033D

    Plasmid

    #211897

    Purpose

    Vinculin (chicken) with S1033 phosphomimetic point mutation (S1033D) tagged with VenusA206K at the C-terminus, in lentiviral expression vector.

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Proc Natl Acad Sci U S A. 2023 Dec 12;12

    Insert

    VinculinVenus-S1033D (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    VenusA206K

    Expression

    Mutation

    mutated vinculin serine 1033 to aspartic acid (S1…

    Promoter

    CMV

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5/FRT/TO HIS DNAJB8

    Plasmid

    #19558

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCEP4-gp120(BaL)-CD4

    Plasmid

    #98957

    Purpose

    Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    HIV-1 (BaL) gp120
    

    Use

    Tags

    CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. PB-Lmo2

    Plasmid

    #60666

    Purpose

    PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Lmo2 (Lmo2 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRSET-pr-mEos4b (mEosFP4b-V69T)

    Plasmid

    #99226

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PIK3CA - Exon9 E545K (pFastBac)

    Plasmid

    #16640

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Baculoviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pEGFP-RapR-FAK-YM-Y397F

    Plasmid

    #25930

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge