We narrowed to 58,603 results for: ran

1. HA tagged Sph ELL2

   Plasmid

   #127270

   Purpose

   For in vitro translation of human WT ELL2 with internal HA tag

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   WT Mets and HA tag at SphI site (8th Met)

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Met138ILeu ELL2

   Plasmid

   #127260

   Purpose

   For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met138 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Met186ILeu ELL2

   Plasmid

   #127261

   Purpose

   For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met186 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met186 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Met138ILeu, Met186ILeu ELL2

   Plasmid

   #127265

   Purpose

   For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Two Mets (138,186) to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Met133ILeu ELL2

   Plasmid

   #127259

   Purpose

   For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met133 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Met133ILeu, Met186ILeu ELL2

   Plasmid

   #127264

   Purpose

   For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Two Mets (133,186 )to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Met331Leu ELL2

   Plasmid

   #127262

   Purpose

   For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met331 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met331 to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Met1ILeu ELL2

   Plasmid

   #127258

   Purpose

   For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met1 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met1 to Ileu by Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ELL2mRNA+polyA

   Plasmid

   #127256

   Purpose

   For in vitro translation of human ELL2 with 30 nt pA tail

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   shortens 3'UTR to 40 bp adds A30 tail

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pMCh1809_pBS_cdc42E6-boxB

    Plasmid

    #118609

    Purpose

    cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E6 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Promoter

    T3

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMCh1807_pBS_cdc42E7-boxB

    Plasmid

    #118612

    Purpose

    cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7 3'UTR (Cdc42 Mouse)
    

    Use

    In vitro transcription

    Tags

    boxB

    Promoter

    T3

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBT3038

    Plasmid

    #122545

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_glo-1::CemOrange2 (glo-1 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBT3043

    Plasmid

    #122546

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24a-VHH-tev-2xTS

    Plasmid

    #117750

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

    Insert

    anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid

    #114135

    Purpose

    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS_cyt-AKAR5

    Plasmid

    #114138

    Purpose

    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5 with C-terminal nuclear export signal (NES)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid

    #114134

    Purpose

    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3_PKA-CαN-mPAGFP

    Plasmid

    #114114

    Purpose

    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCHKU11.1-2.2

    Plasmid

    #115528

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4
    

    Expression

    Yeast

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX6P-1 PUM FL

    Plasmid

    #112997

    Purpose

    For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    pumilio (pum Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLPCX2-HA-GATA3 N1(1-259)

    Plasmid

    #89830

    Purpose

    Express GATA3 N1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    N1 (AA 1-259)

    Promoter

    CMV

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLPCX2-HA-GATA3 C1(295-443)

    Plasmid

    #90316

    Purpose

    Express GATA3 C2 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    GATA3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    C2 (AA 295-443)

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLPCX2-HA-GATA3 N2(1-294)

    Plasmid

    #89831

    Purpose

    Express GATA3 N2 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Mutation

    N2 (AA 1-294)

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(+)mGAT1-565-YFP-566CT

    Plasmid

    #41677

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; YFP inserted d…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1(+)mGAT1-570-CFP-571CT

    Plasmid

    #41680

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; CFP inserted d…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1(+)mGAT1-570-YFP-571CT

    Plasmid

    #41679

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; YFP inserted d…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1(+)mGAT1-565-CFP-566CT

    Plasmid

    #41678

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; CFP inserted d…

    Promoter

    CMV

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti-CMV-mOC-STAMP (N162D)-GFP

    Plasmid

    #73580

    Purpose

    Lentivirus expression of mOC-STAMP with N162D mutation

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    changed asparagin (N) 162 to Aspartic Acid (D)

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pFOSGE_COF3H04

    Plasmid

    #70971

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf6 (Taf6 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pFOSGE_COF1G05

    Plasmid

    #70809

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    CkIIbeta (CkIIbeta Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pFOSGE_COF1B07

    Plasmid

    #70819

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    CkIIalpha (CkIIalpha Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pGL3-IRES-miR34a-P6

    Plasmid

    #64790

    Purpose

    Luciferase expression driven by miR-34a promoter P6

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P6 (MIR34A Human)
    

    Use

    Luciferase

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3-IRES-miR34a-P3

    Plasmid

    #64787

    Purpose

    Luciferase expression driven by miR-34a promoter P3

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P3 (MIR34A Human)
    

    Use

    Luciferase

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGL3-IRES-miR34a-P5

    Plasmid

    #64789

    Purpose

    Luciferase expression driven by miR-34a promoter P5

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P5 (MIR34A Human)
    

    Use

    Luciferase

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

    Plasmid

    #65846

    Purpose

    Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5A-K6A

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

    Plasmid

    #65847

    Purpose

    Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R5D-K6D

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pGL3-IRES-miR34a-P4

    Plasmid

    #64788

    Purpose

    Luciferase expression driven by miR-34a promoter P4

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P4 (MIR34A Human)
    

    Use

    Luciferase

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1(+)mGAT1CFP45

    Plasmid

    #41676

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1(+)mGAT1YFP45

    Plasmid

    #41675

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pM-ErbB4-delta-kinase-PY1 mutant

    Plasmid

    #17801

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Tags

    GAL4-BD

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 988-1292, …

    Available Since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

42. pLDLR-Luc mutSRE

    Plasmid

    #14945

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter mut SRE (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Point mutation in the SRE-1 sequence of the LDL r…

    Available Since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TFORF2973

    Plasmid

    #144449

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARD (PPARD Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

    Plasmid

    #183776

    Purpose

    AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

    Depositor

    Ben Deverman

    Article

    Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

    Insert

    Cre
    

    Use

    AAV

    Mutation

    NA

    Promoter

    CAG

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. AAV-Best1-sCD200

    Plasmid

    #164424

    Purpose

    AAV plasmid expressing soluble CD200 in retinal pigment epithelium

    Depositor

    Connie Cepko

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

    Insert

    CD200 (Cd200 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    deleted terminal 40 amino acids

    Promoter

    Best1

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

    Plasmid

    #216319

    Purpose

    Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

    Depositor

    Elvir Becirovic

    Article

    Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

    Insert

    Split Cerulean + splice acceptor site
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLKO.1_puro_shNRF2-1

    Plasmid

    #230086

    Purpose

    shRNA silencing human NRF2 gene

    Depositor

    Xingguo Zhu

    Article

    Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

    Insert

    Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pKL038 Lenti U6 dCas12a gRNA Puro mCherry

    Plasmid

    #195546

    Purpose

    dCas12a gRNA expression backbone

    Depositor

    Michael Bassik

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Lenti U6- empty cassette_Direct repeat_puro_mcherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. TFORF2314

    Plasmid

    #142029

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RXRB (RXRB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. TFORF3173

    Plasmid

    #144649

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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