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pHH0103_RARA_Hormone-recep

Plasmid

#109836

Purpose

Protein expression and purification of RARA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RARA_Hormone-recep (RARA Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LIC-Z-delCry2

Plasmid

#153545

Purpose

this is the non-clustering version of LIC-Z tagged with mcherry

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

mCherry

Expression

Mammalian

Mutation

with Cry2 sequence deleted from LIC-Z

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-SERCA1

Plasmid

#109552

Purpose

Multisite gateway vector for 3' tagging with sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

sarcoplasmic/endoplasmic calcium ATPase 1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (F126A)-HA

Plasmid

#133067

Purpose

Retroviral expression of mouse MD2 (F126A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (F126A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

F126A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-MD2 (C95A)-HA

Plasmid

#133066

Purpose

Retroviral expression of mouse MD2 (C95A) tagged with HA

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

MD2 (C95A) (Ly96 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

C95A

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-R1597W-VWF

Plasmid

#124826

Purpose

Expresses multimeric human VWF protein without A1 domain and a R1597W mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and R1597W mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-D1498A-VWF

Plasmid

#124825

Purpose

Expresses multimeric human VWF protein without A1 domain and a D1498A mutation in A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and D1498A mutation A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh180

Plasmid

#106838

Purpose

expression of gRNA targeting GYG1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GYG1 (GYG1 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1809_pBS_cdc42E6-boxB

Plasmid

#118609

Purpose

cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E6 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1807_pBS_cdc42E7-boxB

Plasmid

#118612

Purpose

cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 3'UTR (Cdc42 Mouse)

Use

In vitro transcription

Tags

boxB

Promoter

T3

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR141

Plasmid

#106045

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR141; Opto-GPR141; D11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR141 (GPR141 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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gh132

Plasmid

#106791

Purpose

expression of gRNA targeting CHPF2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

CHPF2 (CHPF2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh91

Plasmid

#106762

Purpose

expression of gRNA targeting FUT7

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT7 (FUT7 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh75

Plasmid

#106746

Purpose

expression of gRNA targeting B3GNT9

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GNT9 (B3GNT9 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_T790M_28bp

Plasmid

#114721

Purpose

28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1178

Plasmid

#111104

Purpose

Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1224

Plasmid

#111105

Purpose

Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790M_28bp

Plasmid

#114719

Purpose

4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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APBA1_PDZ_1

Plasmid

#103942

Purpose

Protein expression and purification of APBA1 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

APBA1 (APBA1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Thy-C73A-HA

Plasmid

#111542

Purpose

For expression of human KCNB1 in mouse brain

Depositor

Federico Sesti

Article

Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

Insert

thy1.2-C73A-HA (KCNB1 Human)

Use

Mouse Targeting

Tags

HA

Mutation

C73A

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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