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We narrowed to 5,531 results for: PEP

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Showing: 3921 - 3940 of 5531 results

  2. HA-hDAGLb-V5

    Plasmid
    #87675
    Purpose
    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Insert
    Human Diacylglycerol Lipase Beta (DAGLB Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87675-plasmid-map-sequence-id-173605

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  4. pOCC114

    Plasmid
    #118904
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with TEV protease, two glycines
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118904-plasmid-map-sequence-id-232331

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  5. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Available Since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  12. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  14. pET28a-HsSENP7

    Plasmid
    #71470
    Purpose
    Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 7 (SENP7 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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Showing: 3921 - 3940 of 5531 results