We narrowed to 4,338 results for: Pet2;

1. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. hDHFR

   Plasmid

   #184707

   Purpose

   bacterial expression of human DHFR

   Depositor

   Peter Wright

   Article

   Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

   Insert

   DHFR (DHFR Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-28a-YTH domain of YTHDF3

   Plasmid

   #228777

   Purpose

   Protein expression plasmid for YTH domain of YTHDF3 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF3 (YTHDF3 Human)
   

   Tags

   6*His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a-His6-SUMO-Munc18-1wt

   Plasmid

   #135550

   Purpose

   Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18-1wt
   

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-(-30)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62372

   Purpose

   Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP-9xGGS-Cre-6xHis
   

   Tags

   (-30)GFP and 6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Kindlin

   Plasmid

   #186135

   Purpose

   For expression of recombinant kindlin in bacteria

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   FERMT2 (FERMT2 Human)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHis-hMTMR1

   Plasmid

   #100723

   Purpose

   Expression of human MTMR1 PH-PTP domain

   Depositor

   Byung Il Lee

   Article

   Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

   Insert

   MTMR1 (MTMR1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 95-607

   Promoter

   T7

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcrVIB1

   Plasmid

   #184566

   Purpose

   Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

   Plasmid

   #71755

   Purpose

   His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-LPETG-His
   

   Tags

   6XHis, Sortase (LPETG), and TEV Protease

   Expression

   Bacterial

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIA1363

    Plasmid

    #166861

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp8 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Promoter

    T7-LacO

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA1362

    Plasmid

    #166860

    Purpose

    Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

    Insert

    SARS-COV-2 Nsp7 (ORF1ab Synthetic)
    

    Tags

    His-GB1

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SpyTag003-OspC

    Plasmid

    #159998

    Purpose

    Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    SpyTag003-OspC (20-209) C129S
    

    Tags

    His6-tag and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    C129S mutation improves expression level.

    Promoter

    T7

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. drebrin-His

    Plasmid

    #40363

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Tags

    His and T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pKD077

    Plasmid

    #136473

    Purpose

    SSB-mTur2 IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-mTur2 (ssb E. coli)
    

    Tags

    mTurquoise2

    Expression

    Bacterial

    Mutation

    mTurquoise2 inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available Since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-HISx6-SIMx6

    Plasmid

    #116938

    Purpose

    Bacterial expression vector for 6 sim

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    6 sim
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-HISx6-SUMOx10

    Plasmid

    #116937

    Purpose

    Bacterial expression vector for 10 sumo

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    10 SUMO
    

    Tags

    HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pHsSR beta deltaN Flag

    Plasmid

    #13731

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRPRB Human)
    

    Tags

    6xHis and FLAG

    Expression

    Bacterial

    Mutation

    expresses residues 59-271 of Homo sapiens SRβ wit…

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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