Purpose
targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA
Insert
dCas9-huTET1CD, SgRNA cloning site
Tags
HA-Tag, NLS and T2A-mCherry
Mutation
dCas9 (D10A;H840A)
Promoter
CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
Available Since
Jan. 25, 2021
Availability
Academic Institutions and Nonprofits only