We narrowed to 8,158 results for: Dos;

1. pDG458

   Plasmid

   #100900

   Purpose

   SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-BOS-RIG-I-Myc

   Plasmid

   #215872

   Purpose

   RIG-I-Myc expression vector for overexpression

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

   Insert

   RIG-I (RIGI Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 014LP-pAAV-ANF-657- Cre-pA

   Plasmid

   #120279

   Purpose

   AAV vector for Atrial specific Cre recombinase expression

   Depositor

   Xander Wehrens

   Article

   Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

   Insert

   Cre
   

   Use

   AAV

   Tags

   CRE

   Expression

   Mutation

   Promoter

   ANF promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV_IRES_GFP_CD16a

   Plasmid

   #196189

   Purpose

   Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

   Depositor

   Ana Ruiz-Saenz

   Article

   Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

   Insert

   CD16a (FCGR3A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 158 to Valine

   Promoter

   LTR

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubiquitin (1-76; T66V, L67N)

   Plasmid

   #110755

   Purpose

   Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

   Depositor

   David Komander

   Article

   Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T66V, L67N

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3/V5-DEST-GFP

   Plasmid

   #40125

   Depositor

   Lynda Chin

   Article

   Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

   Insert

   GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRC/CMV-TYK2-VSV

   Plasmid

   #139344

   Purpose

   Expression of TYK2 protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-6xHis-(-30)GFP

   Plasmid

   #62936

   Purpose

   Expression of 6xHis-(-30)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS_3E5E_eGFP

   Plasmid

   #103054

   Purpose

   Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebola virus (ebolavirus Zaire, Mayinga) minigenome
   

   Use

   Tags

   3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAT.000

    Plasmid

    #119559

    Purpose

    Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK Tgfbr3-HA neo

    Plasmid

    #58508

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28c(+)-YTHDF2

    Plasmid

    #102275

    Purpose

    For bacterial expression of His-tagged human YTHDF2

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6X HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_MMP-9-HA

    Plasmid

    #121172

    Purpose

    Expression of mouse MMP9

    Depositor

    M. Elizabeth Fini, Shinwu Jeong

    Article

    Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

    Insert

    MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV immediate/early

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_RAD51_Rad51

    Plasmid

    #110031

    Purpose

    Protein expression and purification of RAD51_Rad51

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RAD51_Rad51 (RAD51 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-iCasp9

    Plasmid

    #199441

    Purpose

    Express inducible Caspase9 in mammalian cells

    Depositor

    Chao Tang

    Article

    Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

    Insert

    inducible Caspase9 (CASP9 Human)
    

    Use

    Lentiviral

    Tags

    DmrB and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CaMKIIa-eGFP-Cre

    Plasmid

    #120219

    Purpose

    Expresses cre under the control of CamKIIa promotor

    Depositor

    Marie Carlén

    Article

    Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

    Insert

    cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1+_Lambda-N-HA-Peptide

    Plasmid

    #92005

    Purpose

    Expresses Lambda-N-HA peptide

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Lambda-N-HA peptide
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 013LP-pAAV-ANF-657-EmGFP-pA

    Plasmid

    #120267

    Purpose

    AAV vector for Atrial specific EmGFP expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    EmGFP
    

    Use

    AAV

    Tags

    3XFlag and HA

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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