We narrowed to 20,388 results for: ACE;

Showing: 3961 - 3980 of 20388 results

Results per page:

Sort results by:

pAAV.hSynapsin.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209664

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Article

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA5/FRT/TO_CalR-HaloTag7-P30-SNAP-tag-KDEL

Plasmid

#182012

Purpose

CMV overexpression of HaloTag7 and SNAP-tag fusion in the endoplasmic reticulum of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

CalR-HaloTag7-P30-SNAP-tag-KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b-PelB-6H-Ag43-160N-sfGFP

Plasmid

#172886

Purpose

Display sfGFP protein on E. coli cell surface

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

SfGFP
Ag43, partial (flu )

Tags

HHHHHH and TEV recognition site (ENLYFQG)

Expression

Bacterial

Mutation

Deleted 1-160 (N terminal)

Promoter

T7 Promoter

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRE5 CTFdeltaTA-FLAG

Plasmid

#217726

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE5 (ADGRE5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-536

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-DIO-GFP-2A-SynmRuby

Plasmid

#166608

Purpose

Encodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GFP-2A-syn

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GW1-mCherryEA

Plasmid

#207867

Purpose

Mammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mCherryEA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AsdCas12a KRAB

Plasmid

#188502

Purpose

Expresses FLAG tagged AsdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Plasmid L5 attB::Pleft* mWasabi

Plasmid

#169409

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mWasabi

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

pLeft
mWasabi

Use

Unspecified

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYSD061

Plasmid

#212915

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-AAVS1-Ef1a-Puro

Plasmid

#227266

Purpose

Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Plasmid

#209728

Purpose

Expresses cytosolic high affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRE5 CTF-FLAG

Plasmid

#217693

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE5 (ADGRE5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-530

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS_FLAG-Mx1201 PylRS

Plasmid

#154764

Purpose

plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCgCas9_recT

Plasmid

#153337

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-TRE-WT human MyoD-T2A-dsRedExpress2

Plasmid

#60628

Purpose

Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD (MYOD1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mEGFP(A206K)-paCaMKII(K42M)

Plasmid

#165440

Purpose

Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(K42M)

Tags

mEGFP(A206K)

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge