We narrowed to 17,600 results for: Jun;

1. CAG.MCAK-mCherry-eDHFR

   Plasmid

   #176204

   Purpose

   MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   MCAK-mCherry-eDHFR
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FKBP-EGFP-PML peptide

   Plasmid

   #175247

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJH858

   Plasmid

   #172176

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]
   

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJH859

   Plasmid

   #172177

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]
   

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJH580

   Plasmid

   #172164

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 3f2v2+B); AraC
   

   Expression

   Bacterial

   Mutation

   PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJH581

   Plasmid

   #172165

   Purpose

   pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Para (pylBCD 3f2+C); AraC
   

   Expression

   Bacterial

   Mutation

   PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJH750

   Plasmid

   #172170

   Purpose

   Microscopy inclusion body assay

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   pCDF.3f2, deleted sumo
   

   Expression

   Bacterial

   Mutation

   Evolved PylBCD operon; SUMO tag deleted from PylB…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJH751

   Plasmid

   #172171

   Purpose

   Microscopy inclusion body assay

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   pCDF.JM10.1, deleted sumo
   

   Expression

   Bacterial

   Mutation

   Evolved PylBCD operon; SUMO tag deleted from PylB…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJH768v2

   Plasmid

   #172173

   Purpose

   PylB overexpression and purification

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   PT7/Lac-[His6-TEV-SUMO-PylB.3f2]
   

   Expression

   Bacterial

   Mutation

   pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJH769v2

    Plasmid

    #172174

    Purpose

    PylB overexpression and purification

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
    

    Expression

    Bacterial

    Mutation

    pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJH61

    Plasmid

    #172143

    Purpose

    Accessory Plasmid for PACE of pylBCD variants

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] pylS; PproK pylT
    

    Expression

    Bacterial

    Mutation

    gIII(P29*); [strong promoter eG, strong RBS SD8] …

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Bax gRNA#1

    Plasmid

    #171827

    Purpose

    Cas9-mediated knockout of Bax in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bax (Bax Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Bax gRNA#2

    Plasmid

    #171828

    Purpose

    Cas9-mediated knockout of Bax in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bax (Bax Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Bax gRNA#3

    Plasmid

    #171829

    Purpose

    Cas9-mediated knockout of Bax in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bax (Bax Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ssh2 gRNA#2

    Plasmid

    #163398

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ssh2 gRNA#1

    Plasmid

    #163397

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ssh2 gRNA#3

    Plasmid

    #163399

    Purpose

    Cas9-mediated knockout of Ssh2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh2 (Ssh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Ssh3 gRNA#2

    Plasmid

    #163401

    Purpose

    Cas9-mediated knockout of Ssh3 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh3 (Ssh3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Ssh3 gRNA#3

    Plasmid

    #163402

    Purpose

    Cas9-mediated knockout of Ssh3 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Ssh3 (Ssh3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAFNF-Cdc42

    Plasmid

    #163606

    Purpose

    Expresses Cdc42 Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Cdc42 (Cdc42 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge