We narrowed to 4,995 results for: mcs

1. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

   Plasmid

   #113864

   Purpose

   Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   PTCH1 (hedgehog dependent promoter)
   mTurquoise2
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV3Tag8li-hTXLNA-HA

   Plasmid

   #165472

   Purpose

   Human TXLNA mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   taxilin alpha (TXLNA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-CRY2-mCh-superPLDx10

   Plasmid

   #188986

   Purpose

   A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Tags

   CRY2 and mCherry

   Expression

   Mammalian

   Mutation

   F163L, K249R, G328S, G381V, G429D, D480G

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

   Plasmid

   #113867

   Purpose

   Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   TOP (wnt dependent promoter)
   NLuc
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-BFP

   Plasmid

   #106282

   Purpose

   A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

   Depositor

   Jay Shendure

   Article

   Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

   Insert

   CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCFJ1272

   Plasmid

   #44486

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV2-SYN-GFP-BGHpA

   Plasmid

   #190229

   Purpose

   Expresses EGFP in neuronal cells

   Depositor

   Hiroyuki Hioki

   Article

   Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-GfaABC1D-DreO-4x6T

   Plasmid

   #196413

   Purpose

   Astrocytic expression of Dre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   DreO
   

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRTCLCrVA.008

    Plasmid

    #22841

    Depositor

    Niki Robertson

    Article

    Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

    Insert

    A DNA (with MCS)
    

    Use

    Cotton vigs vector

    Expression

    Bacterial

    Mutation

    Coat Protein (AR1 gene) of Cotton Leaf Crumple Vi…

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV3Tag8li-hRBM34-HA

    Plasmid

    #165464

    Purpose

    Human RBM34 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    RNA binding motif protein 34 (RBM34 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVEX2.3 TEM-8

    Plasmid

    #16563

    Depositor

    Bert Vogelstein

    Article

    Carson-Walter et al Cancer Res. 2001 Sep 15. 61(18):6649-55.

    Insert

    TEM8 (ANTXR1 Human)
    

    Expression

    Bacterial

    Mutation

    Extracellular region

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-2Aneo

    Plasmid

    #80032

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ1273

    Plasmid

    #44487

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUb LwaCas13a + LwaCas13a Guide RNA

    Plasmid

    #176307

    Purpose

    Expresses LwaCas13a and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    LwaCas13a
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E656G, I1060V, D1076G- please see depositor comme…

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD1122 CMV 3xFLAG-NES-PYL1-VP64 in TUPV3

    Plasmid

    #161547

    Purpose

    Constitutive expression of 3xFLAG-NES-PYL1-VP64 under the CMV promoter

    Depositor

    Joshua Leonard

    Article

    Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

    Insert

    3xFLAG-NES-PYL1-VP64
    

    Use

    Synthetic Biology

    Tags

    3x-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKM53

    Plasmid

    #154156

    Purpose

    Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel1A NF-κB site

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Promoter

    8 copies of the Aedes Aegypti Rel1A NF-κB promoter

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQC V5 HuR.wt IRES Puro

    Plasmid

    #110386

    Purpose

    γ-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Retroviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Neo-PTEN

    Plasmid

    #16411

    Depositor

    Bert Vogelstein

    Article

    Kohli et al Nucleic Acids Res. 2004 . 32(1):e3.

    Insert

    PTEN Neo targeting (PTEN Human)
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only