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pLJM1-zeo-2xStrep-mu68

Plasmid

#162662

Purpose

Lentiviral vector for stable expression of N-terminal 2xStrep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Use

Lentiviral

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-ORF68

Plasmid

#162625

Purpose

Expresses N-terminal 2x-Strep-tagged ORF68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-mu68

Plasmid

#162627

Purpose

Expresses N-terminal 2x-Strep-tagged mu68 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

mu68

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL52

Plasmid

#162628

Purpose

Expresses N-terminal 2x-Strep-tagged UL52 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL52

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-2xStrep-UL32

Plasmid

#162629

Purpose

Expresses N-terminal 2x-Strep-tagged UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Tags

N-terminal 2xStrep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCL21-decoder

Plasmid

#159448

Purpose

Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pact1-lexA-ER-B112-phosphodegron-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

act1

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE048 ZF43x6-S mKate2 (TUPV1)

Plasmid

#138728

Purpose

mMoClo TUPV, with ZF43x6-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Spaced

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE047 ZF43x3-S mKate2 (TUPV1)

Plasmid

#138727

Purpose

mMoClo TUPV, with ZF43x3-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE045 ZF43x6-C mKate2 (TUPV1)

Plasmid

#138725

Purpose

mMoClo TUPV, with ZF43x6-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE044 ZF43x3-C mKate2 (TUPV1)

Plasmid

#138724

Purpose

mMoClo TUPV, with ZF43x3-C promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Compact

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHIE049 ZF43x12-S mKate2 (TUPV1)

Plasmid

#138729

Purpose

mMoClo TUPV, with ZF43x12-S promoter and mKate2 in the MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x12-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x12-Spaced

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.a

Plasmid

#107523

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.a

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_Cdkn1b.b

Plasmid

#107524

Purpose

Negative regulator of cell cycle progression by G1/S transition inhibition

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

Cdkn1b.b

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MTK6_006

Plasmid

#123860

Purpose

Encodes a spacer cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_012

Plasmid

#123704

Purpose

Encodes a spacer as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCCG 306

Plasmid

#131167

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Tags

CCG

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC

Plasmid

#116988

Purpose

Expresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Tolypothrix campylonemoides RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1

Plasmid

#116969

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC

Plasmid

#116970

Purpose

Expresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Micromonospora rosaria RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1

Plasmid

#116981

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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