Addgene

We narrowed to 4,013 results for: GAST

Showing: 11 - 20 of 4013 results

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pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET11d-hUba2

Plasmid

#53136

Purpose

E.coli expression of full length untagged human Uba2 (SAE2)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

UBA2 (UBA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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phGR-SB

Plasmid

#133409

Purpose

overexpression of a human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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GR A458T

Plasmid

#133410

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

A458T

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hGR-Dx

Plasmid

#133408

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Use

Tags

Expression

Mammalian

Mutation

N454D, A458T, R460D, D462C

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-His-hAos1

Plasmid

#53135

Purpose

E.coli expression of full length human Aos1 (SAE1) with Nterminal His-tag

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Aos1 (SAE1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pUG-natNT2

Plasmid

#110922

Purpose

expression of nat gene conferring resistance to nourseothricin for gene deletion in yeast

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

nat

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

AgTEF1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP025

Plasmid

#103874

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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