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  4. pDC146

    Plasmid
    #169366
    Purpose
    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Depositor
    Axel Brunger
    Article
    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45
    Insert
    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    Use
    Tags
    6 his tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169366-plasmid-map-sequence-id-333023

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  6. pGH00.0126

    Plasmid
    #64257
    Purpose
    Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation
    Depositor
    Mark Guiltinan, Siela Maximova
    Article
    Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E
    Inserts
    EGFP
    neomycin phosphotransferase II
    Use
    Binary agrobacterium vector for plant transformat…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    E12-omega
    Available since
    June 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    64257-plasmid-map-sequence-id-115529

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  7. pIMAY

    Plasmid
    #68939
    Purpose
    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange
    Depositor
    Tim Foster
    Article
    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.
    Type
    Empty backbone
    Use
    Staphylococcal allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68939-plasmid-map-sequence-id-210239

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  10. pGU06

    Plasmid
    #212148
    Purpose
    Plasmid expressing alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase and tyrosine aminotransferase under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    alanine-glyoxylate aminotransferase, glutamate-pyruvate aminotransferase, tyrosine aminotransferase (AGX1, alaA, tyrB Agx1: S. cerevisiae; alaA, tyrB: E. coli, Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212148-plasmid-map-sequence-id-420636

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  12. pJK516

    Plasmid
    #71578
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to glutamine
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    71578-plasmid-map-sequence-id-130937

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  13. pJK524

    Plasmid
    #71580
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes asparagine-347 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71580-plasmid-map-sequence-id-130939

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  14. pJK513

    Plasmid
    #71576
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-294 to alanine
    Promoter
    T7
    Available since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71576-plasmid-map-sequence-id-130935

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  15. pJK564

    Plasmid
    #71581
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes serine-71 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71581-plasmid-map-sequence-id-130940

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  16. pJK517

    Plasmid
    #71579
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to aspartate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71579-plasmid-map-sequence-id-130938

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  17. pJK514

    Plasmid
    #71577
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamine-435 to alanine
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71577-plasmid-map-sequence-id-130936

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  18. pJK512

    Plasmid
    #71575
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes arginine-228 to glutamate
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71575-plasmid-map-sequence-id-130934

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  19. pBGE3.0

    Plasmid
    #165988
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA1B1C1
    nourseothricin N-acetyl transferase
    neomycin resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    165988-plasmid-map-sequence-id-378724

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  20. pAGE2.0

    Plasmid
    #165984
    Purpose
    Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymA origin and Tet resistance.
    Depositor
    Bogumil Karas
    Article
    Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do
    Inserts
    RK2/RP4 origin of transfer
    repA2B2C2
    nourseothricin N-acetyl transferase
    tetracycline resistance
    Use
    Synthetic Biology; Bacterial and yeast cloning; c…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    165984-plasmid-map-sequence-id-375867

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Showing: 21 - 40 of 5094 results