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pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

Plasmid

#146166

Purpose

Insect Expression of DmUPF2-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF2-siRNAres (Upf2 Fly)

Use

Tags

Expression

Insect

Mutation

3 non silent mutations: L1022P, V1097L and N1213S…

Promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-CFP

Plasmid

#74151

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-CFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

Plasmid

#184549

Purpose

Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Retroviral

Tags

3xFLAG and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

Plasmid

#187844

Purpose

Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Use

Tags

Ig k-chain leader sequence, SNAPf-tag, and meGFPe…

Expression

Mammalian

Mutation

meGFPe: asparagine 198 to aspartic acid and tyros…

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-dsRed

Plasmid

#74152

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-dsRed

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

Plasmid

#192785

Purpose

Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNAR1 (IFNAR1 Human)

Use

Tags

Ig k-chain leader sequence, SNAPf-tag, and mXFPe

Expression

Mammalian

Mutation

mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-sfGFP

Plasmid

#74153

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker sfGFP

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-sfGFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-del119-272-siRNAres_P

Plasmid

#147194

Purpose

Mammalian Expression of HsUpf1-del119-272-siRNAres

Depositor

Elisa Izaurralde

Insert

HsUpf1-del119-272-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations A69S, K282R, A442G and T1…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-kanaR-cryaa-YFP

Plasmid

#74154

Purpose

BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

Tol2-kanaR-cryaa-YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HsSmg6-del36-576-siRNAres_I

Plasmid

#146553

Purpose

Mammalian Expression of HsSmg6-del36-576-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6-del36-576-siRNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCA21-HSPB1 5'HA-NarTag (12XPP7)-3'HA (HDR donor)

Plasmid

#199452

Purpose

CRISPR donor plasmid to insert NarTag (mTagBFP harbors 12XPP7 in its intron) into the C-terminus of human HSPB1 gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCA29-pCS2-CMV-Zebrafish NarTag (eGFP)

Plasmid

#199460

Purpose

Expression of Zebrafish NarTag (eGFP harbors 9XMS2 in its intron) in zebrafish embryos

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

Zebrafish NarTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNAres_AH

Plasmid

#148927

Purpose

Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del975-1031GSSG-dsRNAres_Y

Plasmid

#148069

Purpose

Insect Expression of DmPAN2-del975-1031GSSG-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del975-1031GSSG-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-dsRNAres_Y

Plasmid

#148063

Purpose

Insect Expression of DmPAN2-del130-133G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del130-133G-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148065

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del358-467-dsRNAres_Y

Plasmid

#148066

Purpose

Insect Expression of DmPAN2-del358-467-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del358-467-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del468-981-dsRNAres_Y

Plasmid

#148067

Purpose

Insect Expression of DmPAN2-del468-981-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del468-981-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-del486-493GSG-dsRNAres_Y

Plasmid

#148068

Purpose

Insect Expression of DmPAN2-del486-493GSG-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del486-493GSG-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-del130-133G-dsRNAres_Y

Plasmid

#148032

Purpose

Insect Expression of DmPAN2-del130-133G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del130-133G-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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