We narrowed to 842 results for: golden gate assembly

1. ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

   Plasmid

   #195049

   Purpose

   integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   NAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pPGI

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. (K) tNOS (S)_T3 (SBE120)

   Plasmid

   #195010

   Purpose

   terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (K) tNOS (S)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. (H) tNOS (I) _T2 (SBE119)

   Plasmid

   #195006

   Purpose

   terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (H) tNOS (I)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. (B) HYG marker(R) (SBE264)

   Plasmid

   #195013

   Purpose

   hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+HYG+tGPD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXYL

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. (E) tNOS (F) _T1 (SBE118)

   Plasmid

   #194997

   Purpose

   terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (E) tNOS (F)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. (B) BLE marker(R) (SBE262)

   Plasmid

   #195012

   Purpose

   bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   pXYL+BLE+tGPD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pXYL

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. (R)pGPD1(D)_P1(SBE109)

   Plasmid

   #194993

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P1 position (overhangs R/D)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (R) pGPD (D)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. (F)pGPD(G)_P2 (SBE110)

   Plasmid

   #194992

   Purpose

   promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P2 position (overhangs F/G)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (F) pGPD (G)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. (I) pADH2 (J) P3 (SBE114)

   Plasmid

   #187629

   Purpose

   promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the P3 position (I/J)

   Depositor

   Petri-Jaan Lahtvee

   Article

   Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

   Insert

   (I) pADH2 (J)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. (F) pADH2 (G)_P2 (SBE113)

    Plasmid

    #187628

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides fro the P2 position (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pADH2 (G)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

    Plasmid

    #195054

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLDP1

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

    Plasmid

    #195053

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFBA

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

    Plasmid

    #195052

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

    Plasmid

    #195051

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pGPD

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

    Plasmid

    #195050

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pADH2

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (F) pXYL (G) P1 (SBE216)

    Plasmid

    #195017

    Purpose

    promoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (F) pXYL (G)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. (R) pXYL (D) P1 (SBE215)

    Plasmid

    #195016

    Purpose

    promoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (R) pXYL (D)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. (R)pADH2(D)_P1(SBE214)

    Plasmid

    #195015

    Purpose

    promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (B) pADH2 (R)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. (B) NAT marker (R) (SBE173)

    Plasmid

    #195014

    Purpose

    nourseothricin (NAT) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tNOS

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    pXYL+NAT+tNOS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pXYL

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (I) pGPD (J)_P3 (SBE111)

    Plasmid

    #194990

    Purpose

    promoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (I) pGPD (J)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge