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pLEX-EF1a ANXA11-mEmerald D40G

Plasmid

#164211

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

D40G

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R346C

Plasmid

#164213

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

R346C

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-EF1a ANXA11-mEmerald R235Q

Plasmid

#164212

Purpose

pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

R235Q

Promoter

EF1a

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-ER WPRE

Plasmid

#236230

Purpose

AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)

Use

AAV

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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Talin-T48-L432G-Cterminal-mEmerald

Plasmid

#202373

Purpose

Talin(DeltaR2-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-10)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T58-L432G-Cterminal-mEmerald

Plasmid

#202372

Purpose

Talin(DeltaR4-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T100-L432G-Cterminal-mEmerald

Plasmid

#202371

Purpose

Talin(FullLength)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(FullLength)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-T46-L432G-Cterminal-mEmerald

Plasmid

#202374

Purpose

Talin(DeltaR4-12)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-12)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-Sec61b WPRE

Plasmid

#236228

Purpose

AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Sec61b (SEC61B Human, Synthetic)

Use

AAV

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mEmerald-STIM1 WPRE

Plasmid

#236234

Purpose

AAV expression of mouse STIM1 internally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mEmerald-Stim1 (Stim1 Mouse)

Use

AAV

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Expression

Mutation

Promoter

U6 and human Synapsin1

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

Plasmid

#236241

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV1.2 (CACNA1C Rabbit)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

Plasmid

#236239

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.1 (CACNA1A Rabbit)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

Plasmid

#236240

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV2.2 (CACNA1B Rabbit)

Use

Lentiviral

Tags

mEmerald

Expression

Mutation

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR-mEm

Plasmid

#181990

Purpose

MiniR genetically encoded tag on H2B-mEmerald to be used with MiniE labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniR-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-MiniE-mEm

Plasmid

#181991

Purpose

MiniE genetically encoded tag on H2B-mEmerald to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-CoilE-mEm

Plasmid

#181992

Purpose

CoilE genetically encoded tag on H2B-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

H2B-CoilE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-CoilE-mEm

Plasmid

#181994

Purpose

CoilE genetically encoded tag on Mito-mEmerald, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

Mito-CoilE-mEm

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_670

Plasmid

#100460

Purpose

Mammalian expression of E. coli LF82_670 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_670

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge