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We narrowed to 682 results for: pCMV-HA

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Showing: 381 - 400 of 682 results

  1. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    160140-plasmid-map-sequence-id-317331

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  5. pCMV6-AN-HA_WT POLI

    Plasmid
    #201440
    Purpose
    Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells
    Depositor
    Roger Woodgate
    Publication
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNA polymerase iota (POLI Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Coding sequence has been optimised for expression…
    Promoter
    CMV
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201440-plasmid-map-sequence-id-394929

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  6. pCMV-eGFP-mPlum-HA

    Plasmid
    #200009
    Purpose
    Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.
    Depositor
    Thomas Noll
    Publication
    Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP, mPlum
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200009-plasmid-map-sequence-id-398431

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  7. pCMV-ADARcd-YTHmut

    Plasmid
    #194431
    Purpose
    ADAR1 catalytic domain E488Q fused to YTHmut
    Depositor
    Kate Meyer
    Publication
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADARcd fused to YTHmut domain (ADAR Human)
    Tags
    ADARcd-YTHmut-HA
    Expression
    Mammalian
    Mutation
    m6A binding region of YTH domain is deleted
    Available since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194431-plasmid-map-sequence-id-382431

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  10. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

    Plasmid
    #185916
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8.20m-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185916-plasmid-map-sequence-id-367354

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  17. pcmv3-IRG1-H159Q-HA

    Plasmid
    #198183
    Purpose
    Mammalian expression of HA-tagged human ACOD1 (IRG1) H159Q
    Depositor
    Yun Zhao
    Publication
    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACOD1 (ACOD1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    The histidine in IRG1 at position 159 mutates to …
    Promoter
    CMV
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198183-plasmid-map-sequence-id-389088

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  18. pcmv3-IRG1-L272Q-HA

    Plasmid
    #198184
    Purpose
    Mammalian expression of HA-tagged human ACOD1 (IRG1) L272Q
    Depositor
    Yun Zhao
    Publication
    Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACOD1 (ACOD1 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    The leucine in IRG1 at position 272 mutates to gl…
    Promoter
    CMV
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198184-plasmid-map-sequence-id-389089

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Showing: 381 - 400 of 682 results