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We narrowed to 78,865 results for: TRI

Showing: 5971 - 6000 of 78865 results

  3. pC-G418-YR

    Plasmid
    #61767
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    nptII gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Tags
    Expression
    Mutation
    Promoter
    trpC promoter
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61767-plasmid-map-sequence-id-367929

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  6. hHSF1 RNAi resistant

    Plasmid
    #118347
    Purpose
    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).
    Depositor
    Lea Sistonen
    Article
    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.
    Insert
    hHSF1 (HSF1 Human)
    Use
    Tags
    myc and 6xHIS
    Expression
    Mammalian
    Mutation
    RNAi resistance mutations (does not change amino …
    Promoter
    Available since
    Nov. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    118347-plasmid-map-sequence-id-227547

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  14. pALS2-sfGFP 150TAG

    Plasmid
    #197574
    Purpose
    Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    araC and lpp
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    197574-plasmid-map-sequence-id-388126

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  16. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Use
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  17. pOPINB-hRNF216(457-784)

    Plasmid
    #171922
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171922-plasmid-map-sequence-id-346327

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  19. p5E-Runx1+23

    Plasmid
    #69602
    Purpose
    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning
    Depositor
    Owen Tamplin, Leonard Zon
    Article
    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:
    Inserts
    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    Use
    5' gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    69602-plasmid-map-sequence-id-190603

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  20. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  25. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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Showing: 5971 - 6000 of 78865 results