We narrowed to 7,286 results for: 11

1. pKM50

   Plasmid

   #123656

   Purpose

   Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

   Depositor

   Kevin Maringer

   Article

   Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

   Insert

   Aedes aegypti UbL40 promoter
   

   Use

   Luciferase

   Expression

   Insect

   Promoter

   UbL40 promoter

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pODN-mR2-MYH10

   Plasmid

   #183869

   Purpose

   Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 homology arms with mRuby2-linker (MYH10 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

   Plasmid

   #61966

   Purpose

   Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

   Depositor

   Lynne Regan

   Article

   Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

   Inserts

   N-mCherry-ME(pS)VD
   TRAP(RK)-C-mCherry
   

   Expression

   Bacterial

   Promoter

   PBAD and PLtetO

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQFlag-USP11 CS puroR

   Plasmid

   #46748

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-HypaCas9-ACTB_sgRNA

   Plasmid

   #183885

   Purpose

   pX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD_Ub11TCA

   Plasmid

   #174517

   Purpose

   Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   Ubiquitin
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Recoded with TCA replacing codon 11 of wt ubiquit…

   Promoter

   araBAD

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyso-AktAR2

   Plasmid

   #64933

   Purpose

   Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-AktAR2 (LAMP1 Budding Yeast, Human, Synthetic)
   

   Tags

   Cerulean3, Full-length LAMP1, and cpVenus[E172]

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-26

   Plasmid

   #172751

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-26
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-ubiquitin-SV40-GFP

    Plasmid

    #65445

    Purpose

    Expression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectors

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    SV40-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UBQ and SV40

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. MTK0_051

    Plasmid

    #123948

    Purpose

    Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hAAVS1 landing pad destination vector
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Tollip M240A,F241A

    Plasmid

    #178051

    Purpose

    GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

    Depositor

    David Tumbarello

    Article

    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

    Insert

    Toll-Interacting Protein (TOLLIP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 240 to Alanine and changed Phe…

    Promoter

    CMV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK0_003

    Plasmid

    #123925

    Purpose

    Encodes the spCas9 cassette and spCas9 gRNA GFP dropout expression cassette final destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Cas9-sgRNA destination
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_Human_SAT1_Cterm_His

    Plasmid

    #220487

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_Human_SAT1_Nterm_His

    Plasmid

    #220482

    Purpose

    heterologous expression of SSAT1 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    SSAT1 (SAT1 Human)
    

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. QSOX1-luciferase

    Plasmid

    #113352

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    QSOX1-enhancer (QSOX1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-21

    Plasmid

    #172749

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-21; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-21
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-37

    Plasmid

    #172756

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-37
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpeCas9-HF

    Plasmid

    #192141

    Purpose

    Expresses highly specific SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9-HF
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    SpeCas9-HF (R247A, N415A, S421A)

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. iRFP713-N2-Frankenbody-Flag

    Plasmid

    #175291

    Purpose

    Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

    Depositor

    Tim Stasevich

    Article

    Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

    Insert

    anti-FLAG frankenbody fused with iRFP713
    

    Tags

    iRFP713

    Expression

    Mammalian

    Available Since

    Dec. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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