We narrowed to 7,281 results for: 11

1. QSOX1-Tol2

   Plasmid

   #113383

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   QSOX1-enhancer (QSOX1 Human)
   

   Use

   Tol2 reporter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pET21-pelB-LaG-29

   Plasmid

   #172753

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-29
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT5T_hp53_ST

   Plasmid

   #34922

   Depositor

   Thanos Halazonetis

   Article

   Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

   Insert

   p53 DNA-binding (Res 1-393) (TP53 Human)
   

   Expression

   Bacterial

   Mutation

   C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

   Promoter

   T7

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJB556

   Plasmid

   #115966

   Purpose

   SGP insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 -98/15 SGP 2xTetApt-STOP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459-HypaCas9-ECT2_sgRNA

   Plasmid

   #183873

   Purpose

   pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ECT2 sgRNA spacer (ECT2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Hygro-rKIF5B-GFP

   Plasmid

   #210335

   Purpose

   Overexpression of rKIF5B

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   rKIF5B (Kif5b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-ubiquitin-SV40-RFP

   Plasmid

   #65448

   Purpose

   Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   SV40-RFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBQ

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pmEos2-C1-centrin-1

   Plasmid

   #72588

   Purpose

   mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):

   Insert

   centrin 1 (CETN1 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB3035(X-4 MarkerFree)

   Plasmid

   #73273

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-AMPKsub(TA)-YPet-NES

    Plasmid

    #84636

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub(TA)-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK0_057

    Plasmid

    #123950

    Purpose

    Encodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI LP on hAAVS1 (Hygro::mRuby)
    

    Expression

    Mammalian

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK0_047

    Plasmid

    #123977

    Purpose

    Encodes the Cas9 hCLYBL homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    hCLYBL CAS9 Destination - HygroR
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 Flag-Smad1 (4SP/AP+AAVA)

    Plasmid

    #14955

    Depositor

    Joan Massague

    Article

    Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

    Insert

    Smad1 (4SP/AP+AAVA) (SMAD1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Four Serine to Alanine mutations in the linker re…

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

16. 374 pME-ets1a

    Plasmid

    #49000

    Purpose

    full length coding sequence for zebrafish ets1a gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    v-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
    

    Use

    Gateway middle entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB3034(X-3 MarkerFree)

    Plasmid

    #73272

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMX-SHP1C-ires-hCD8

    Plasmid

    #183720

    Purpose

    Expresses the myc-tagged catalytic domains of wild-type SHP-1.

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

    Insert

    SHP-1
    

    Use

    Retroviral

    Mutation

    catalitic domain alone

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pODN-AAVS1-mR2-CAAX

    Plasmid

    #183871

    Purpose

    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-CAAX

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Promoter

    CMV

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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